Summary
Indocyanine green (ICG) obeyed the Beer-Lambert law within the concentration range 1.25 μg/ml–10.0μg/ml in distilled water, methanol, dimethylformamide (DMF), 1.2-propanediol and aqueous buffers (pH 9.0), but only up to 7.5 μg/ml in human bile and 0.5% human albumin, and only to 5.0 μg/ml in human duodenal fluid. ICG was rapidly (<1 h) decomposed to a colorless derivative at pH<5 and >11, but remained relatively stable for 48 h at pH 8–10. ICG is an indicator and a weak acid with a pKa of 3.27. In bile stabilized with 25% methanol, the precision of the method (CV) is 5% and the accuracy is 106–127%.
This is a preview of subscription content, log in via an institution to check access.
References
Brooker, L.G.S., Experientia, suppl.2, 229 (1955).
Fox, I.J., and Wood, E.H., Proc. Staff Meet. Mayo Clin.35, 732 (1960).
Fox, I.J., Brooker, L.G.S., Heseltine, D.W., and Wood, E.H., Circulation14, 937 (1956).
Cherrick, G.R., Stein, S.W., Leevy, C.M., and Davidson, C.S., J. clin. Invest.39, 592 (1960).
Caesar, J., Shaldon, S., Chiandussi, L., Guevara, L., and Sherlock, S., Clin. Sci.21, 43 (1961).
Leevy, C.M., Mendenhall, C.L., Lesko, W., and Howard, M.M., J. clin. Invest.41, 1169 (1962).
Reubi, F.C., Vorburger, C., Pfeiffer, G., and Golder, S., Clin. Sci. molec. Med.51, 151 (1976).
Saunders, K.B., Hoffman, J.I.E., Noble, M.I.M., and Domenech, R.J., J. appl Physiol.28, 190 (1970).
Ketterer, S.G., Wiegand, B.D., and Rapaport, E., Am. J. Physiol.199, 481 (1960).
Vogin, E.E., Moreno, O.M., Brodie, D.A., Mattis, P.A., and Scott, W.K., J. appl. Physiol.21, 1880 (1966).
Hunton, D.B., Bollman, J.L., and Hoffman, H.N., Gastroenterology39, 713 (1960).
Klaassen, C.G., and Plaa, G.L., Toxic. appl. Pharmac.15, 374 (1969).
Paumgartner, G., Probst, P., Kraines R., and Leevy, C.M., Ann. N.Y. Acad. Sci. USA170, 134 (1970).
Paumgartner, G., Schweiz. med. Wschr., suppl.105, 1 (1975).
van Berge Henegouwen, G.P., and Hofmann, A.F., Gastroenterology75, 879 (1978).
Björnsson, Ó.G., Adrian, T.E., Dawson, J., McCloy, R.F., Greenberg, G.R., Bloom, S.R., and Chadwick, V.S., Eur. J. clin. Invest.9, 293 (1979).
Barbier, F., and de Weerdt, G.A., Clinica chim. Acta10, 549 (1964).
Michie, D.D., Goldsmith, R.S., and Mason, A.D., Proc. Soc. exp. Biol. Med.111, 540 (1962).
Sutterer, W.F., Hardin, S.E., Benson, R.W., Krovetz, L.J., and Schiebler, G.L., Am. Heart J.72, 345 (1966).
Gathje, J., Steuer, R.R., and Nicholes, K.R.K., J. appl. Physiol.29, 181 (1970).
Landsman, M.L.J., Kwant, G., Mook, G.A., and Zijlstra, W.G., J. appl. Physiol.40, 575 (1976).
Porstmann, W., and Banaschak, H., Dte Gesundh Wes.20, 889 (1965).
Dawson, R.M.C., Elliott, D.C., Elliott, W.H., and Jones, K.M., eds., Data for biochemical research, 2nd edn, p. 502. Clarendon Press, Oxford 1978.
Flexser, L.A., Hammett, L.P., and Dingwall, A., J. Am. chem. Soc.57, 2103 (1935).
Jones Owen, V.M., Clin. Biochem.6, 132 (1973).
Baker, K.J., Proc. Soc. exp. Biol. Med.122, 957–963 (1966).
Björnsson, Ó.G., Khan, R.A.A., Murphy, R., and Chadwick, V.S., Clin. Res.28, 272A (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Björnsson, Ó.G., Murphy, R. & Chadwick, V.S. Physicochemical studies of indocyanine green (ICG): absorbance/concentration relationship, pH tolerance and assay precision in various solvents. Experientia 38, 1441–1442 (1982). https://doi.org/10.1007/BF01955757
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01955757