PT  - JOURNAL ARTICLE
AU  - T. Yu
AU  - X. Huang
AU  - S. Jiang
AU  - K. Hu
AU  - B. Kong
AU  - Z. Wang
TI  - Ultrastructure alterations in adriamycin-resistant and cisplatin-resistant human ovarian cancer cell lines exposed to nonlethal ultrasound
AID  - 10.1136/ijgc-00009577-200505000-00008
DP  - 2005 Apr 01
TA  - International Journal of Gynecologic Cancer
PG  - 462--467
VI  - 15
IP  - 3
4099  - http://ijgc.bmj.com/content/15/3/462.short
4100  - http://ijgc.bmj.com/content/15/3/462.full
SO  - Int J Gynecol Cancer2005 Apr 01; 15
AB  - Human ovarian cancer cell lines, SKOV3 and its adriamycin-resistant substrain SKOV3/ADR and COC1 and its cisplatin-resistant substrain COC1/DDP, were exposed to nonlethal ultrasound. Ultrastructures in sham-insonated and insonated cells were inspected by transmission electron microscopy, and cytochrome C in cytosol was determined by enzyme-linked immunosorbent assay. Ultrasound exposure led to no significant changes in SKOV3/ADR cells, but tumid mitochondria occurred in SKOV3 cells. Mitochondria changes were also detected in some exposed COC1 and COC1/DDP cells. Apoptotic bodies could be detected in either control or insonated COC1/DDP cells. A few exposed COC1/DDP cells became reticular. Cytochrome C in cytosol in exposed SKOV3/ADR cells was increased but that in exposed COC1/DDP cells was decreased. These findings revealed that the bioeffect of ultrasound on chemosensitive cells was not identical to that of chemoresistant ones, and ultrasound was a potential approach for treatment of drug-resistant ovarian cancers.