RT Journal Article SR Electronic T1 Profiling of differentially expressed genes in human uterine leiomyomas JF International Journal of Gynecologic Cancer JO Int J Gynecol Cancer FD BMJ Publishing Group Ltd SP 146 OP 154 DO 10.1136/ijgc-00009577-200501000-00022 VO 15 IS 1 A1 E.-J. Lee A1 G. Kong A1 S.-H. Lee A1 S. B. Rho A1 C.-S. Park A1 B.-G. Kim A1 D.-S. Bae A1 J. J. Kavanagh A1 J.-H. Lee YR 2005 UL http://ijgc.bmj.com/content/15/1/146.abstract AB Uterine leiomyomas are very common benign tumors resulting in clinically serious gynecological problems in women of reproductive age. Approximately, 1% of leiomyosarcoma was reported to arise in a preexisting leiomyoma. However, the molecular basis of these tumors is poorly understood. To understand the molecular changes during leiomyoma development, we profiled differentially expressed genes in ten paired leiomyoma and normal myometrial tissues using cDNA microarray chip analysis. We identified 67 genes (27 overexpressed and 40 underexpressed) which were scored as differentially expressed at least twofold in at least eight of ten patients. Eighteen of 67 genes have been already reported to be differentially expressed without their established functions in uterine leiomyoma and others have never been reported. Subsequently, the relative expression levels of representative genes from identified 67 genes were confirmed by reverse-transcriptase polymerase chain reaction and immunohistochemistry and were found to be consistent with the microarray data. This study could provide a new insight into the understanding of leiomyoma and leiomyosarcoma.