TY - JOUR T1 - Alterations of the P16 gene in uterine cervical carcinoma from Indian patients JF - International Journal of Gynecologic Cancer JO - Int J Gynecol Cancer SP - 472 LP - 479 DO - 10.1136/ijgc-00009577-200307000-00011 VL - 13 IS - 4 AU - A. Tripathi (Bhar) AU - S. Banerjee AU - A. Roy AU - S. Roychowdhury AU - C. K. Panda Y1 - 2003/06/01 UR - http://ijgc.bmj.com/content/13/4/472.abstract N2 - In our analysis, alterations in the P16 tumor suppressor gene were seen in 33% (15/46) of sampled uterine cervical lesions. Among the alterations, mutations in P16 were detected in 15% (7/46) of the samples. One mutation occurred at intron 1/exon 2 splice junction. All the other mutations were in exon 2 with three of them as silent mutations. The promoter hypermethylation and homozygous deletion of P16 gene were detected in 6.5% (3/46) and 8.7% (4/46) of the samples respectively. Loss of heterozygosity and microsatellite size alterations at the P16 locus were seen in 17% (8/46) of the samples. HPV16/18 infection was detected in 76% (35/46) of the samples. But no association was found between P16 alterations and HPV infection. Thus, it seems that P16 inactivation may be associated with the development of some uterine cervical carcinoma. ER -