@article {Koshiba208, author = {Hisato Koshiba and Kenichi Hosokawa and Akiko Kubo and Norimasa Tokumitsu and Ai Watanabe and Hideo Honjo}, title = {Junctional Adhesion Molecule: An Expression in Human Endometrial Carcinoma}, volume = {19}, number = {2}, pages = {208--213}, year = {2009}, doi = {10.1111/IGC.0b013e31819bc6e9}, publisher = {BMJ Specialist Journals}, abstract = {Junctional adhesion molecule A (JAM-A) is involved in cell-cell contact and tight junction formation. Loss of cell adhesion molecules may be associated with high histologic grade and invasiveness of endometrial carcinoma. We attempted to determine JAM-A expression in human endometrial carcinoma and its correlations with pathologic features, stage, and survival. Junctional adhesion molecule A expression in human endometrial carcinoma was evaluated by immunohistochemistry. In addition, we cultured human well and poorly differentiated endometrial adenocarcinoma cell lines, Ishikawa cells, and KLE in 3-dimensional basement membrane preparation, and JAM-A expression in these cells was assessed by real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Junctional adhesion molecule A immunostaining intensity was negatively correlated with histologic grade (τ = -0.420, P \< 0.0001), myometrial invasion (τ = -0.306, P \< 0.01), and stage (τ = -0.383, P \< 0.0001). Low JAM-A immunostaining intensity was associated with positive vascular space involvement (P \< 0.01). Moreover, low immunostain intensity was significantly (P \< 0.0001) related to low overall survival rate and progression-free survival rate. Additionally, in our 3-dimensional epithelial cell culture, JAM-A expression in poorly differentiated adenocarcinoma was significantly lower than that in well-differentiated adenocarcinoma (P \< 0.001). Junctional adhesion molecule A expression seems to be reduced in high-grade or advanced endometrial carcinoma and may be a prognostic factor.}, issn = {1048-891X}, URL = {https://ijgc.bmj.com/content/19/2/208}, eprint = {https://ijgc.bmj.com/content/19/2/208.full.pdf}, journal = {International Journal of Gynecologic Cancer} }