TY - JOUR T1 - Annexin IV is Differentially Expressed in Clear Cell Carcinoma of the Ovary JF - International Journal of Gynecologic Cancer JO - Int J Gynecol Cancer SP - 1545 LP - 1549 DO - 10.1111/IGC.0b013e3181a844c8 VL - 19 IS - 9 AU - Yi Miao AU - Bin Cai AU - Ling Liu AU - Yixia Yang AU - Xiaoping Wan Y1 - 2009/11/01 UR - http://ijgc.bmj.com/content/19/9/1545.abstract N2 - Objective: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray.Methods: Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC.Results: Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray.Conclusions: The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.Abbreviations: CCC- clear cell carcinoma, SAC- serous carcinoma, PCR- polymerase chain reaction, RT-PCR- reverse transcriptase PCR, ABCF2- ATP-binding cassette, sub-family F- member 2, HNF-1b- hepatocyte nuclear factor 1β ER -