PT  - JOURNAL ARTICLE
AU  - Yi Miao
AU  - Bin Cai
AU  - Ling Liu
AU  - Yixia Yang
AU  - Xiaoping Wan
TI  - Annexin IV is Differentially Expressed in Clear Cell Carcinoma of the Ovary
AID  - 10.1111/IGC.0b013e3181a844c8
DP  - 2009 Nov 01
TA  - International Journal of Gynecologic Cancer
PG  - 1545--1549
VI  - 19
IP  - 9
4099  - http://ijgc.bmj.com/content/19/9/1545.short
4100  - http://ijgc.bmj.com/content/19/9/1545.full
SO  - Int J Gynecol Cancer2009 Nov 01; 19
AB  - Objective: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray.Methods: Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC.Results: Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray.Conclusions: The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.Abbreviations: CCC- clear cell carcinoma, SAC- serous carcinoma, PCR- polymerase chain reaction, RT-PCR- reverse transcriptase PCR, ABCF2- ATP-binding cassette, sub-family F- member 2, HNF-1b- hepatocyte nuclear factor 1β