PT  - JOURNAL ARTICLE
AU  - J. Y. Song
AU  - J. K. Lee
AU  - N. W. Lee
AU  - H. H. Jung
AU  - S. H. Kim
AU  - K. W. Lee
TI  - Microarray analysis of normal cervix, carcinoma <em>in situ</em>, and invasive cervical cancer: identification of candidate genes in pathogenesis of invasion in cervical cancer
AID  - 10.1111/j.1525-1438.2007.01164.x
DP  - 2008 Aug 01
TA  - International Journal of Gynecologic Cancer
PG  - 1051--1059
VI  - 18
IP  - 5
4099  - http://ijgc.bmj.com/content/18/5/1051.short
4100  - http://ijgc.bmj.com/content/18/5/1051.full
SO  - Int J Gynecol Cancer2008 Aug 01; 18
AB  - The objective of this study was to identify genes that are related to pathogenesis of carcinoma in situ (CIS) to invasive cervical cancer with the use of oligonucleotide microarray and reverse transcription-polymerase chain reaction (RT-PCR). Each two cases of normal cervix, CIS, and invasive cervical cancer were investigated with DNA microarray technology. Differential gene expression profiles among them were analyzed. Expression levels of selected genes from the microarray results were confirmed by RT-PCR. The expressions of 15,286 genes were compared and 458 genes were upregulated or downregulated by twofold or more compared with each other group. Among 458 genes, 22 genes were upregulated and 40 genes were downregulated by twofold or more in invasive cervical cancer group compared with CIS group. RT-PCR analysis confirmed upregulation of 18 genes and downregulation of 5 genes in invasive cervical cancer group. RBP1, TFRC, SPP1, SAA1, ARHGAP8, and NDRG1, which were upregulated, and GATA3, PLAGL1, APOD, DUSP1, and CYR61, which were downregulated, were considered as candidate genes associated with invasion of cervical cancer.