RT Journal Article
SR Electronic
T1 Placenta-Specific Protein 1 Expression in Human Papillomavirus 16/18–Positive Cervical Cancers Is Associated With Tumor Histology
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP 784
OP 790
DO 10.1097/IGC.0000000000000957
VO 27
IS 4
A1 Eric J. Devor
A1 Henry D. Reyes
A1 Jesus Gonzalez-Bosquet
A1 Akshaya Warrier
A1 Susan A. Kenzie
A1 Nonye V. Ibik
A1 Marina D. Miller
A1 Brandon M. Schickling
A1 Michael J. Goodheart
A1 Kristina W. Thiel
A1 Kimberly K. Leslie
YR 2017
UL http://ijgc.bmj.com/content/27/4/784.abstract
AB Objective Expression of the trophoblast-specific gene placenta-specific protein 1 (PLAC1) has been detected in a wide variety of cancers. However, to date, PLAC1 expression has not been shown in cervical cancer. We have carried out a preliminary study that shows for the first time that PLAC1 is expressed in cervical cancers.Methods A total of 16 primary cervical tumors were obtained from patients shown to be human papillomavirus (HPV) 16/18 positive. Total cellular RNA, genomic DNA, and total protein were purified from each tumor. These materials were then used to determine PLAC1 expression, TP53 mutation status, and p53 expression.Results The PLAC1 expression was demonstrated in all 16 primary cervical tumors. The highest levels of expression were found in the more aggressive squamous and adenosquamous histologic types compared with adenocarcinomas. Moreover, the proportion of total PLAC1 message coming from the P1 promoter, also termed the distal or cancer promoter, was significantly greater in the more aggressive squamous and adenosquamous histologic types compared with adenocarcinomas. Finally, in spite of all 16 tumors being HPV-16/18 positive, 3 of 8 squamous cell cancers and 2 of 5 adenocarcinomas expressed wild-type p53 protein. Consistent with the recently shown suppression of the PLAC1P1 promoter by wild-type p53, these p53 positive tumors displayed among the lowest P1-specific PLAC1 expression levels.Conclusions The PLAC1 expression has been demonstrated for the first time in cervical cancers. This preliminary study has further revealed a complex relationship between PLAC1 expression, cervical cancer histologic type, p53, and HPV type that requires further investigation.