PT  - JOURNAL ARTICLE
AU  - Quan Zhou
AU  - Ling R. Han
AU  - Yang X. Zhou
AU  - Yan Li
TI  - MiR-195 Suppresses Cervical Cancer Migration and Invasion Through Targeting Smad3
AID  - 10.1097/IGC.0000000000000686
DP  - 2016 Jun 01
TA  - International Journal of Gynecologic Cancer
PG  - 817--824
VI  - 26
IP  - 5
4099  - http://ijgc.bmj.com/content/26/5/817.short
4100  - http://ijgc.bmj.com/content/26/5/817.full
SO  - Int J Gynecol Cancer2016 Jun 01; 26
AB  - Objective MicroRNAs (miRNAs) play crucial roles in cervical cancer development and progression. The purposes of this study were to investigate the role of miR-195 in cervical cancer and clarify the regulation of Smad3 by miR-195.Methods Quantitative real-time polymerase chain reaction was used to examine miR-195 expression in cervical cancer tissues and cell lines. The clinicopathological significance of miR-195 down-regulation was further analyzed. Transwell migration and invasion assays were performed. A luciferase reporter assay was conducted to confirm the target gene of miR-195, and the results were validated in cervical cancer tissues and cell lines.Results MiR-195 was significantly decreased in clinical tissues and cervical cancer cell lines. The low miR-195 level was significantly correlated with higher International Federation of Gynecology and Obstetrics stage, node metastasis, and deep stromal invasion. Up-regulation of miR-195 suppressed cell migration and invasion in vitro. Smad3 was verified as a direct target of miR-195, which was further confirmed by the inverse expression of miR-195 and Smad3 in patients’ specimens.Conclusions The newly identified miR-195/Smad3 pathway provides an insight into cervical cancer metastasis and may represent a novel therapeutic target.