PT  - JOURNAL ARTICLE
AU  - Hyun Joo Lee
AU  - Hye-Jung Choi
AU  - Heung-Mo Yang
AU  - You Min Kim
AU  - Jeeyun Lee
AU  - Dongil Chio
AU  - BoKyung Kim
AU  - Yoon-La Choi
AU  - Sung Joo Kim
TI  - Establishment of Primary Xenograft Model From Newly Characterized Patient Extrauterine Carcinosarcoma
AID  - 10.1097/01.IGC.0000434105.98035.c7
DP  - 2013 Nov 01
TA  - International Journal of Gynecologic Cancer
PG  - 1552--1560
VI  - 23
IP  - 9
4099  - http://ijgc.bmj.com/content/23/9/1552.short
4100  - http://ijgc.bmj.com/content/23/9/1552.full
SO  - Int J Gynecol Cancer2013 Nov 01; 23
AB  - Background and Objective The aim of this study was to characterize primary cells from extrauterine carcinosarcoma (CS) and to establish a primary CS xenograft mouse model.Methods Primary cells were isolated from a patient with CS and cultured in vitro. Primary CS cells were verified for their ability to consecutively generate tumorigenesis in NOD/SCID mice. The properties of xenograft tumor and explants cells were investigated by immunohistochemistry, cytogenetic, and FACS analysis. Anticancer drug susceptibility of primary CS was analyzed using CCK-8.Results Primary CS cells greater than 27 passages in vitro showed an ability of a series of xenograft tumorigenesis in vivo having the same marker expression and cytogenetic character as that of original tumor. In addition, explants of xenograft tumors retained their original characteristics in the in vitro culture system. Finally, the analysis of the susceptibility to anticancer drug revealed that primary CS cells were susceptible to both doxorubicin and nilotinib, which are tyrosine kinase inhibitors.Conclusions The primary CS cells and the primary CS xenograft tumorigenesis introduce a new therapeutic model for targeting cancer and also explore a deeper understanding of generation of the tumor itself.