PT  - JOURNAL ARTICLE
AU  - Zhengyu Li
AU  - Wenjiao Min
AU  - Canhua Huang
AU  - Shujun Bai
AU  - Minghai Tang
AU  - Xia Zhao
TI  - Proteomics-Based Approach Identified Differentially Expressed Proteins With Potential Roles in Endometrial Carcinoma
AID  - 10.1111/IGC.0b013e3181a9026d
DP  - 2010 Jan 01
TA  - International Journal of Gynecologic Cancer
PG  - 9-15--9-15
VI  - 20
IP  - 1
4099  - http://ijgc.bmj.com/content/20/1/9-15.short
4100  - http://ijgc.bmj.com/content/20/1/9-15.full
SO  - Int J Gynecol Cancer2010 Jan 01; 20
AB  - Instruction: We used proteomic approaches to identify altered expressed proteins in endometrial carcinoma, with the aim of discovering potential biomarkers or therapeutic targets for endometrial carcinoma.Methods: The global proteins extracted from endometrial carcinoma and normal endometrial tissues were separated by 2-dimensional electrophoresis and analyzed with PDQuest (Bio-Rad, Hercules, Calif) software. The differentially expressed spots were identified by mass spectrometry and searched against NCBInr protein database. Those proteins with potential roles were confirmed by Western blotting and immunohistochemical assays.Results: Ninety-nine proteins were identified by mass spectrometry, and a cluster diagram analysis indicated that these proteins were involved in metabolism, cell transformation, protein folding, translation and modification, proliferation and apoptosis, signal transduction, cytoskeleton, and so on. In confirmatory immunoblotting and immunohistochemical analyses, overexpressions of epidermal fatty acid-binding protein, calcyphosine, and cyclophilin A were also observed in endometrial carcinoma tissues, which were consistent with the proteomic results.Conclusions: Our results suggested that these identified proteins, including epidermal fatty acid-binding protein, calcyphosine, and cyclophilin A, might be of potential values in the studies of endometrial carcinogenesis or investigations of diagnostic biomarkers or treatment targets for endometrial carcinoma.