RT Journal Article
SR Electronic
T1 Gene Expression Profiling of Endometrial Adenocarcinomas Reveals Increased Apolipoprotein E Expression in Poorly Differentiated Tumors
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP 1226-1231
OP 1226-1231
DO 10.1111/IGC.0b013e3181b33be0
VO 19
IS 7
A1 Jutta Huvila
A1 Annika Brandt
A1 Clarissa Rios Rojas
A1 Salla Pasanen
A1 Lauri Talve
A1 Pirkko HirsimÄKi
A1 Vidal Fey
A1 Leena KytÖMÄKi
A1 Pekka Saukko
A1 Olli CarpÉN
A1 Juhani T. Soini
A1 Seija GrÉNman
A1 Annika Auranen
YR 2009
UL http://ijgc.bmj.com/content/19/7/1226-1231.abstract
AB Introduction: Tumor grade is one of the most important prognostic factors in endometrioid endometrial adenocarcinoma. Amplification of oncogenes, such as Her2/neu, or loss of function of tumor suppressor genes, such as p53, are known to be associated with poor prognosis, but additional factors influencing clinical behavior are likely to exist. To examine the biological differences between low-grade and high-grade endometrioid endometrial adenocarcinomas, we compared gene expression in these 2 types of tumors.Methods: Six well-differentiated adenocarcinomas and 7 poorly differentiated adenocarcinomas were studied with 2 different microarray platforms, Affymetrix and Illumina. The expression of the most differentially expressed gene on both platforms was further studied in 34 endometrial adenocarcinoma samples (10 well differentiated, 9 moderately differentiated, and 15 poorly differentiated) using real-time reverse transcription-polymerase chain reaction.Results: The most differentially expressed gene on both platforms was Apolipoprotein E (APOE). In the poorly differentiated adenocarcinomas, APOE was overexpressed 13.1-fold (P = 0.001) and 9.7-fold (P = 0.007) when compared with well- and moderately differentiated tumors, respectively. There was no difference in APOE expression between well- and moderately differentiated adenocarcinomas.Conclusions: Increased expression of APOE might represent a late event in the progression of well-differentiated endometrioid endometrial adenocarcinoma to a poorly differentiated endometrioid endometrial adenocarcinoma. Although increased APOE expression has been previously reported in other malignancies, this is the first study to suggest that APOE might also have a role in endometrioid endometrial cancer.