@article {Tang898-904, author = {Binzhi Tang and Yi Qu and Fengyan Zhao and Meng Mao and Jun Tang and Xihong Li and Donna Ferriero and Dezhi Mu}, title = {In Vitro Effects of Hypoxia-Inducible Factor 1α on the Biological Characteristics of the SiHa Uterine Cervix Cancer Cell Line}, volume = {19}, number = {5}, pages = {898-904--898-904}, year = {2009}, doi = {10.1111/IGC.0b013e3181a14f9a}, publisher = {BMJ Specialist Journals}, abstract = {Introduction: Hypoxia-inducible factor 1α (HIF-1α) regulates the transcription of many genes involved in key aspects of cancer biology. The aim of our study was to explore the effects of HIF-1α on the biological characteristics of the uterine cervix cancer (UCC) cell line SiHa, such as proliferation, apoptosis, and migration under normoxia and hypoxia.Methods: Full-length HIF-1α (fL HIF-1α) and dominant-negative HIF-1α (dn HIF-1α) were transfected into UCC SiHa cells. The expression of HIF-1α and its targets such as vascular endothelial growth factor (VEGF), CXC chemokine receptor 4 (CXCR4), and human growth and transformation-dependent protein (HGTD-P) was detected by immunocytochemistry, Western blot, and semiquantitative reverse transcription-polymerase chain reaction. Cell proliferation, apoptosis, and migration were surveyed by methyl thiazolyl tetrazolium assay, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling staining, and scratching test.Results: The expression of HIF-1α increased in fL HIF-1α but not in dn HIF-1α SiHa cells. Consistently, the expression of HIF-1α target genes such as VEGF, CXCR 4 , and HGTD-P increased in fL HIF-1α-transfected SiHa cells but decreased in dn HIF-1α-transfected SiHa cells. The UCC cells transfected with fL HIF-1α had increased cellular proliferation and migration. However, the inhibition of HIF-1α through dn HIF-1α attenuated cell proliferation and migration under both normoxia and hypoxia.Conclusions: Hypoxia-inducible factor 1α affects the proliferation, apoptosis, and migration of UCC SiHa cells in part by regulating the expression of its target genes such as VEGF, HGTD-P, and CXCR4 . Targeting HIF-1α may be a promising strategy for molecular therapy for UCC.}, issn = {1048-891X}, URL = {https://ijgc.bmj.com/content/19/5/898-904}, eprint = {https://ijgc.bmj.com/content/19/5/898-904.full.pdf}, journal = {International Journal of Gynecologic Cancer} }