RT Journal Article
SR Electronic
T1 #270 Immunophenotypic features and BRCA1/2 mutations from high-grade serous cancer: the role of cytopathological assessment
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP A265
OP A265
DO 10.1136/ijgc-2023-ESGO.550
VO 33
IS Suppl 3
A1 Miceska, Simona
A1 škof, Erik
A1 Novakovic, Srdjan
A1 Stegel, Vida
A1 Smrkolj, Špela
A1 Cvijeticanin, Branko
A1 Jericevic, Anja
A1 Kuzmanov, Biljana Grcar
A1 Prevodnik, Veronika Kloboves
YR 2023
UL http://ijgc.bmj.com/content/33/Suppl_3/A265.1.abstract
AB Introduction/Background High-grade serous cancer (HGSC) is the most common and aggressive type of ovarian cancer, and it is often associated with ascites at presentation. The objective of our research was to evaluate the accuracy of cytopathology to identify immunophenotypic features of HGSC and BRCA1/2 mutations from ascites.Methodology Forty-five patients with histologically confirmed primary HGSC and malignant ascites were included in our study. Immunocytochemistry (ICC) staining for PAX8, WT1, P53, P16, and Ki-67 was performed on cytospins and cytoblocks prepared from ascites. Next-generation sequencing (NGS) was used to detect germline/somatic BRCA1/2 mutations in the ascites. Both ICC and NGS results were compared with immunohistochemistry (IHC) and NGS results from tissue blocks of the primary tumor. Cronbach α and χ2 statistics, respectively, were used.Results ICC/IHC results for PAX8, WT1, P53, and P16 showed good reliability between cytospins, cytoblocks, and tissue blocks (α > 0.75), whereas poor reliability and significant differences were observed for Ki-67 between ascites and tissue blocks (α < 0.26; p < .001 [Kruskal-Wallis]). For germline BRCA1/2 mutations, 100% concordance was confirmed, but only 14% concordance was confirmed for somatic mutations.Conclusion Our results confirmed that cytopathology is an accurate method for identifying immunophenotypic features of HGSC and detecting germline BRCA1/2 mutations from ascites. However, further investigation is required for assessing the proliferation activity of HGSC in ascites and for detecting somatic BRCA1/2 mutations.Abstract #270 Figure 1 WT1, PAX8, P53, P16, Ki67 positive stained tumor cells on ascites cytospins and cell blocks, and tumor tissue blocks (immunhisto/cytochemistry results). 200x magnification.Disclosures The authors made no disclosures. The study was funded by the Slovenian Research Agency (research program P3–0289).