PT  - JOURNAL ARTICLE
AU  - Jian jun Wang
AU  - Ying Zheng
AU  - Fang Yang
AU  - Ping Zhao
AU  - Huai fang Li
TI  - Survivin Small Interfering RNA Transfected With a Microbubble and Ultrasound Exposure Inducing Apoptosis in Ovarian Carcinoma Cells
AID  - 10.1111/IGC.0b013e3181c5ddfa
DP  - 2010 May 01
TA  - International Journal of Gynecologic Cancer
PG  - 500-506--500-506
VI  - 20
IP  - 4
4099  - http://ijgc.bmj.com/content/20/4/500-506.short
4100  - http://ijgc.bmj.com/content/20/4/500-506.full
SO  - Int J Gynecol Cancer2010 May 01; 20
AB  - Introduction: Small interfering RNA (siRNA) has been used to knock down the expression of specific genes. However, its delivery remains a challenge. Recently, ultrasound combined with microbubbles has been used to deliver plasmid into cells and has shown much superiority. Whether a survivin siRNA transfected with a microbubble contrast agent combined with ultrasound exposure could inhibit survivin expression and induce ovarian cancer cell apoptosis was investigated.Methods: The survivin gene was amplified and inserted into vector pEGFP-N1, resulting in psurvivin-EGFP. Three siRNA expression cassettes (SECs) targeting survivin were obtained by 2-step polymerase chain reaction. The SECs and psurvivin-EGFP were cotransfected into 293T cells. A functional SEC was selected and inserted into pMD18T, resulting in the survivin-targeting siRNA expression plasmid, which was transfected into SKOV-3 ovarian carcinoma cells using SonoVue, a microbubble contrast agent, and ultrasound exposure. Survivin expression was monitored by Western blot, and apoptosis was determined by fluorescence-activated cell sorting.Results: One siRNA effectively inhibited survivin expression. SonoVue with ultrasound effectively delivered survivin siRNA to SKOV-3 cells, inhibited survivin expression, and induced apoptosis.Conclusions: Delivery of survivin siRNA using a microbubble contrast agent combined with ultrasound exposure can effectively inhibit survivin expression and induce apoptosis, providing a new promising approach for siRNA delivery in vivo.