PT  - JOURNAL ARTICLE
AU  - Wen-Fei Wei
AU  - Ling-Fei Han
AU  - Dan Liu
AU  - Lan-Fang Wu
AU  - Xiao-Jing Chen
AU  - Hong-Yan Yi
AU  - Xiang-Guang Wu
AU  - Mei Zhong
AU  - Yan-hong Yu
AU  - Li Liang
AU  - Wei Wang
TI  - Orthotopic Xenograft Mouse Model of Cervical Cancer for Studying the Role of MicroRNA-21 in Promoting Lymph Node Metastasis
AID  - 10.1097/IGC.0000000000001059
DP  - 2017 Oct 01
TA  - International Journal of Gynecologic Cancer
PG  - 1587--1595
VI  - 27
IP  - 8
4099  - http://ijgc.bmj.com/content/27/8/1587.short
4100  - http://ijgc.bmj.com/content/27/8/1587.full
SO  - Int J Gynecol Cancer2017 Oct 01; 27
AB  - Abstract Cervical cancer is the most frequent cause of gynecologic cancer–associated death worldwide. Animal models that demonstrate metastatic patterns consistent with the clinical course of cervical cancer are urgently needed to conduct studies focused on understanding the mechanisms of the disease and identifying optimal treatments. To address this, we established an orthotopic xenograft model of cervical cancer in female NOD-SCID mice using SiHa and ME180 cell lines stably expressing green fluorescent protein to evaluate the role of microRNA-21 (miR-21) in spontaneous lymph node metastasis in vivo. In this case, SiHa and ME180 cells were transduced by lentivirus to stably express green fluorescent protein and miR-21. Overexpression of miR-21 promoted proliferation, migration, and invasion of SiHa and ME180 cells in vitro. Finally, an orthotopic xenograft model of human cervical cancer was successfully established in NOD-SCID mice. Using this model, we confirmed that overexpression of miR-21 resulted in an increase in the size of primary tumors and in the frequency of spontaneous lymph node metastasis at the time of excision. Therefore, the use of the orthotopic xenograft model should allow for the investigation of novel factors that affect metastasis of cervical cancer and presents an opportunity to evaluate potential therapeutic agents that may inhibit the spread of the disease.