RT Journal Article
SR Electronic
T1 Prognostic Impact of the Cancer Stem Cell–Related Marker NANOG in Ovarian Serous Carcinoma
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP 1489
OP 1496
DO 10.1097/IGJ.0b013e3182738307
VO 22
IS 9
A1 Maria Lee
A1 Eun Ji Nam
A1 Sang Wun Kim
A1 Sunghoon Kim
A1 Jae Hoon Kim
A1 Young Tae Kim
YR 2012
UL http://ijgc.bmj.com/content/22/9/1489.abstract
AB Objective The objective of this study was to evaluate the prognostic significance of NANOG expression in ovarian serous carcinoma.Methods The expression of NANOG was evaluated in 6 ovarian carcinoma cell lines, paclitaxel-resistant SKOV3 cells, and SKOV3 spheroid cells with semiquantitative reverse transcription–polymerase chain reaction and Western blotting. NANOG expression was also measured immunohistochemically in a tissue microarray containing ovarian tissues from 74 patients with ovarian serous carcinoma and 24 with ovarian serous cystadenoma. Each sample was scored based on signal intensity and proportion, and a score greater than 4 was considered “positive.”Results NANOG mRNA expression was variable in different ovarian cancer cell lines. The mRNA level of NANOG was increased in the paclitaxel-resistant SKOV3 cells and SKOV3 spheroid cells compared with that in the SKOV3 cells. NANOG expression was positive in 21.6% of 74 ovarian serous carcinoma tissues, but none of the ovarian serous cystadenoma tissues were positive. Positive NANOG expression was associated with residual tumor size after surgery (P = 0.032). The overall survival of the patients with positive NANOG expression was poorer than that of the patients with negative NANOG expression (P = 0.020). In patients with stage I and II disease, positive NANOG expression was independently associated with shorter overall survival compared with negative NANOG expression (40 vs 120 months, respectively; P = 0.031).Conclusions Positive NANOG expression is associated with poor prognosis of ovarian serous carcinoma. NANOG has potential as a predictor of survival for patients with ovarian carcinomas and may be involved in the mechanism of chemoresistance.