PT  - JOURNAL ARTICLE
AU  - Xiao-Yu, Pan
AU  - Yan, Jin
AU  - Cui-Ping, Feng
AU  - Ya-Nan, Wang
AU  - Hua, Lin
AU  - Hua-Jun, L
TI  - Altered Claudin-4 Expression in Progesterone-Treated Endometrial Adenocarcinoma Cell Line Ishikawa
AID  - 10.1097/IGC.0b013e318272ab12
DP  - 2012 Nov 01
TA  - International Journal of Gynecologic Cancer
PG  - 1585--1590
VI  - 22
IP  - 9
4099  - http://ijgc.bmj.com/content/22/9/1585.short
4100  - http://ijgc.bmj.com/content/22/9/1585.full
SO  - Int J Gynecol Cancer2012 Nov 01; 22
AB  - Objective To detect the expression change of claudin-4 in Ishikawa endometrial adenocarcinoma cell line in response to progesterone. To determine whether claudin-4 is involved in the anticancer effect of progesterone.Methods 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to determine the 50% inhibitory concentration (IC50) of megestrol acetate (MA) in treating Ishikawa cells. After the Ishikawa cells were treated with MA at IC50, cell apoptosis was examined by flow cytometry and transmission electron microscopy. The messenger RNA and protein expression levels of claudin-4 were further quantified by real-time polymerase chain reaction and Western blot. The localization of claudin-4 was examined by immunofluorescent staining.Results The IC50 of MA on Ishikawa cells was 15 mg/L incubated for 72 hours. Apoptosis percentage was elevated from 0.07% ± 0.02% to 3.93% ± 0.81% after MA treatment. The expression of claudin-4 at both protein and messenger RNA levels was significantly decreased after the treatment of MA (P < 0.05). The localization of claudin-4 transferred from cytomembrane to cytoplasm and nucleus.Conclusion Megestrol acetate can inhibit the growth of Ishikawa cells. It may work through decreasing claudin-4 expression and cell apoptosis. The localization change of claudin-4 may also be involved in the anticancer effect of progesterone.