PT  - JOURNAL ARTICLE
AU  - Chen, Xiaoxiang
AU  - Liu, Xiufang
AU  - Wang, Jingmei
AU  - Guo, Wenwen
AU  - Sun, Caixia
AU  - Cai, Zhenming
AU  - Wu, Qiang
AU  - Xu, Xia
AU  - Wang, Yaping
TI  - Functional Polymorphisms of the <em>hOGG1</em> Gene Confer Risk to Type 2 Epithelial Ovarian Cancer in Chinese
AID  - 10.1097/IGC.0b013e31823122c6
DP  - 2011 Nov 01
TA  - International Journal of Gynecologic Cancer
PG  - 1407--1413
VI  - 21
IP  - 8
4099  - http://ijgc.bmj.com/content/21/8/1407.short
4100  - http://ijgc.bmj.com/content/21/8/1407.full
SO  - Int J Gynecol Cancer2011 Nov 01; 21
AB  - Objective: 8-Hydroxydeoxyguanosine (8-OHdG) is an oxidized nucleoside that can lead to misincorporation of bases. Human 8-oxoguanine DNA glycosylase (hOGG1) is the key defense enzyme against mutation by the cellular 8-OHdG in duplex DNA. The present study was aimed to explore whether the hOGG1 gene variants play an important role in the carcinogenesis of epithelial ovarian cancer (EOC).Methods: Germ line variants in 5′-untranslated region (c.-18G>T, c.-23A>G, c.-45G>A, and c.-53G>C) and c.977C>G (Ser326Cys) polymorphism in exon7 of the hOGG1 gene in 420 sporadic EOCs and 840 controls were detected. Immunohistochemical and promoter luciferase activity assays were used to explore the effect of c.-18G>T variant on hOGG1 expression.Results: In contrast to type I EOC cases, patients with type II EOC were usually older, already in the advanced stage, and exhibited a common protein 53 (p53) overexpression. The frequencies of genotypes c.-18G/T and c.977G/G in hOGG1 were significantly high in the patients with type II EOC (odds ratio, 2.83; 95% confidence interval, 1.45-5.52; odds ratio, 1.66; 95% confidence interval, 1.26-2.17) but not in the patients with type I EOC. The average level of hOGG1 protein in the normal tissues adjacent to the type II EOC-carried c.-18G/T was lower than that with c.-18G/G (P = 0.01). The luciferase activity in the c.-18T allele was lower than that in the c.-18G allele (P = 0.001).Conclusion: The genotypes of c.-18G/T in 5′-untranslated region and c.977G/G in exon7 of the hOGG1 gene would confer risk to type II EOC.