RT Journal Article
SR Electronic
T1 Regulation of Ovarian Cancer Cell Adhesion and Invasion by Chloride Channels
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP 526-530
OP 526-530
DO 10.1111/IGC.0b013e3181a3d6d2
VO 19
IS 4
A1 Min Li
A1 Qing Wang
A1 Wei Lin
A1 Bo Wang
YR 2009
UL http://ijgc.bmj.com/content/19/4/526-530.abstract
AB The purpose of this study was to investigate the effects of chloride (Cl−) channels on the adhesive and invasive potentials of human ovarian cancer cell line A2780. By using the adhesion and Transwell invasion assays, we showed that 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (200 μmol/L), a nonselective Cl− channel blocker, significantly inhibits cancer cell adhesion and invasion. 5-Nitro-2-(3-phenylpropylamino)-benzoate (200 μmol/L), niflumic acid (100 μmol/L), and tamoxifen (30 μmol/L) had similar inhibitory effects. Regulatory volume decrease is markedly suppressed by 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid, 5-nitro-2-(3-phenylpropylamino)-benzoate, niflumic acid, and tamoxifen. Moreover, intracellular calcium concentration ([Ca2+]i) measurements indicated that a Cl− channel-mediated increase in [Ca2+]i is also one of the mechanisms of cancer cell adhesion and invasion. Our results strongly suggest that Cl− channels may regulate ovarian cancer cell adhesion and invasion, probably through inducing a regulatory volume decrease and mediating a [Ca2+]i increase.