RT Journal Article
SR Electronic
T1 EP280 Role of ganglioside GM1 expression in T lymphocytes membranes in cervical cancer development
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP A209
OP A209
DO 10.1136/ijgc-2019-ESGO.341
VO 29
IS Suppl 4
A1 D Danolic
A1 M Heffer
A1 J Wagner
A1 I Skrlec
A1 I Alvir
A1 I Mamic
A1 L Susnjar
A1 L Marcelic
A1 T Becejac
A1 M Puljiz
YR 2019
UL http://ijgc.bmj.com/content/29/Suppl_4/A209.1.abstract
AB Introduction/Background Cervical cancer is worldwide the second most common cancer in women. Immune response appears to be a key determinant in controlling human papilloma virus (HPV) infection. Cell mediated immunity plays a significiant role in progression or regression of neoplastic cervical lesions. Lipid rafts have essential role in T lymphocite activation. Gangliosides play an important role in the formation and stabilization of specific cell lipid membrane domains. This study aims to determine whether ganglioside GM1 expressions in T cell membrane represent risk factor for cervical cancer.Methodology In total, 20 women participated in our study. Peripheral blood samples were obtained from 10 cervical cancer patients and 10 healthy controls at University Hospital for Tumors, Zagreb, Croatia. A 3 mL sample of venous blood was collected from each subject into a test tube containing EDTA as anticoagulant. For T cell subtypes isolation we used Anti CD4 Phycoerythrin antibody and Anti CD8 Peridinin Chlorophyll Protein Complex antibody. Ganglioside GM1 expression in T lymphocytes membranes was analyzed using double labeling antibodies on ganglioside GM1.Results We have not found difference in ganglioside GM1 expression in T lymphocytes membranes between the two groups.Conclusion The present study did not show association of ganglioside GM1 expression in T lymphocytes membranes with cervical cancer development.Disclosure Nothing to disclose.