RT Journal Article
SR Electronic
T1 EP471 Molecular analysis of p53 codon 72 polymorphism among women with endometrial cancer in morocco: a case-control study
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP A298
OP A298
DO 10.1136/ijgc-2019-ESGO.530
VO 29
IS Suppl 4
A1 B Amal
A1 B Mustapha
A1 EK Mohammed
A1 E Moulay
YR 2019
UL http://ijgc.bmj.com/content/29/Suppl_4/A298.2.abstract
AB Introduction/Background This study aims to investigate whether the genotype distribution of p53 codon 72 may be a risk factor for endometrial cancer among Moroccan women.Methodology Samples from 20 Moroccan women, 6 diagnosed with endometrial cancer and 14 healthy controls were used. HPV detection was done by nested PCR with MY09/11 and GP5+/6+ primers followed by sequencing for HPV genotyping. p53 codon 72 polymorphism determination was performed by allele-specific PCR assay. Viral DNA was detected in 16,6% of cases and 14,2% of control. HPV 16 was the most predominant in cancer and controls cases.Results The distribution of Arg/Arg, Arg/Pro and Pro/Pro genotypes was 33.2%, 49.8% and 16.6% in the endometrial cancer group and 21.4%, 71,4% and 7,14% in the control group.Conclusion No significant association was found between polymorphism of p53 itself as well as in combination with HPV16/18 infection and risk of development of endometrial cancer among Moroccan women. Thus, the polymorphism of p53 codon 72 in exon 4 does not seem to play a role in the development of endometrial cancer among Moroccan women.Disclosure Nothing to disclose.