PT  - JOURNAL ARTICLE
AU  - B Amal
AU  - B Mustapha
AU  - EK Mohammed
AU  - E Moulay
TI  - EP471 Molecular analysis of p53 codon 72 polymorphism among women with endometrial cancer in morocco: a case-control study
AID  - 10.1136/ijgc-2019-ESGO.530
DP  - 2019 Nov 01
TA  - International Journal of Gynecologic Cancer
PG  - A298--A298
VI  - 29
IP  - Suppl 4
4099  - http://ijgc.bmj.com/content/29/Suppl_4/A298.2.short
4100  - http://ijgc.bmj.com/content/29/Suppl_4/A298.2.full
SO  - Int J Gynecol Cancer2019 Nov 01; 29
AB  - Introduction/Background This study aims to investigate whether the genotype distribution of p53 codon 72 may be a risk factor for endometrial cancer among Moroccan women.Methodology Samples from 20 Moroccan women, 6 diagnosed with endometrial cancer and 14 healthy controls were used. HPV detection was done by nested PCR with MY09/11 and GP5+/6+ primers followed by sequencing for HPV genotyping. p53 codon 72 polymorphism determination was performed by allele-specific PCR assay. Viral DNA was detected in 16,6% of cases and 14,2% of control. HPV 16 was the most predominant in cancer and controls cases.Results The distribution of Arg/Arg, Arg/Pro and Pro/Pro genotypes was 33.2%, 49.8% and 16.6% in the endometrial cancer group and 21.4%, 71,4% and 7,14% in the control group.Conclusion No significant association was found between polymorphism of p53 itself as well as in combination with HPV16/18 infection and risk of development of endometrial cancer among Moroccan women. Thus, the polymorphism of p53 codon 72 in exon 4 does not seem to play a role in the development of endometrial cancer among Moroccan women.Disclosure Nothing to disclose.