RT Journal Article
SR Electronic
T1 EP774 Gallic acid exhibits lower cytotoxicity on normal ovarian cells than cisplatin-sensitive and cisplatin-resistant ovarian cancer cells
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP A430
OP A430
DO 10.1136/ijgc-2019-ESGO.825
VO 29
IS Suppl 4
A1 N Al Balushi
A1 N Abdulla
A1 B Al Dhahli
A1 I Hasan
A1 S Dobretsov
A1 S Al Bahlani
A1 B Tsang
A1 Y Tamimi
A1 I Burney
YR 2019
UL http://ijgc.bmj.com/content/29/Suppl_4/A430.1.abstract
AB Introduction/Background Ovarian cancer is one of the fatal cancers in women, often associated with drug resistance. Therefore, finding effective drugs, including naturally derived compounds is urgently needed. We have previously examined the effect of gallic acid (GA) on breast cancer cell line MCF-7 and found a significant anti-cancer activity. We aimed to test the anti-cancer potential of GA monohydrate and six different derivatives of GA on cisplatin-sensitive (A2780S) and cisplatin-resistant (A2780CP) human epithelial ovarian cancer cell lines and on HOSE6-3 normal epithelial ovarian cell line. The effect of cisplatin and GA in combination was also examined.Methodology Cells were exposed to different concentration of GA (0–100 µg/ml), and cisplatin (0–24 µg/ml) and cell viability was tested via AlamarBlue and CCK08 assays. Apoptosis was determined by Hoechst stain.Results GA monohydrate decreased cell viability in a dose- and time-dependent manner in all cell lines. GA monohydrate was demonstrated to have the highest cytotoxicity to both A2780S and A2780CP cell lines compared to all six derivatives. The highest cytotoxicity of GA was observed in A2780S (IC-50=19.4 µg/ml), followed by A2780CP cells (IC-50=35.6 µg/ml), whereas the least cytotoxicity was observed in HOSE6-3 (IC-59=49.3 µg/ml). Similarly, cisplatin displayed a higher cytotoxicity on A2780S (IC-50=9.4 µg/ml) than on A2780CP (IC-50=23.1 µg/ml). However, cisplatin was more toxic to HOSE6-3 than ovarian cancer cells (IC-50=8.8 µg/ml). There was no significant difference in cytotoxicity between cisplatin and GA either combined or used separately. Apoptosis was estimated to be 49% and 47% in A2780S and A2780CP cell lines respectively at a GA concentration of 25 µg/ml.Conclusion GA monohydrate exhibited significant cytotoxicity in ovarian cancer cell lines independent of cisplatin, suggesting a cytotoxic effect of GA on ovarian cancer. The growth inhibitory effect of GA, especially on resistant ovarian cancer cells, merits further investigation.Disclosure We are greatly thankful for His Majesty Sultan Qaboos Trust Fund Grant (SR/MED/MEDE/17/01). Also, we would like to thank Ms Buthaina Al Dhahli for her endless support and help.