RT Journal Article
SR Electronic
T1 EP265 The role of methylation silencing of tumor suppressor genes in cervical HSIL: a prospective cytologic-histologic correlation study of 70 cases
JF International Journal of Gynecologic Cancer
JO Int J Gynecol Cancer
FD BMJ Publishing Group Ltd
SP A202
OP A203
DO 10.1136/ijgc-2019-ESGO.326
VO 29
IS Suppl 4
A1 O Ondic
A1 J Bouda
A1 J Nemcova
A1 I Kinkorova-Lunackova
A1 J Chytra
A1 J Presl
A1 R Alaghehbandan
A1 K Cerna
A1 B Gomolcakova
A1 J Kostun
A1 H Sidlova
A1 P Vlasak
A1 O Majek
YR 2019
UL http://ijgc.bmj.com/content/29/Suppl_4/A202.3.abstract
AB Introduction/Background Methylation silencing of tumor suppressor genes has been suggested as one of the epigenetic changes promoting carcinogenesis. The aim of this study was to prospectively evaluate methylation status of CADM 1, MAL, hsa-miR-124 genes in HSIL (high grade squamous intraepithelial lesion) LBC (liquid based cytology) samples with histologic correlation.Methodology 70 histologically confirmed cases of HSIL paired with prior screening LBC diagnosis of HSIL within 3 months interval were selected. Histologically, the lesions were reviewed and assessed including 1) number of blocks harboring dysplastic squamous epithelium, 2) number of blocks containing glandular extension of dysplastic epithelium, and 3) the depth of glandular extension (which was assessed semi-quantitatively as graded 1-3). From residual LBC materials, HPV subtyping was performed, using LINEAR ARRAY HPV Genotyping Test and in house PCR targeting HPV E1 gene. Detection of methylation silencing of tumor suppressor genes CADM1, MAL, and hsa-miR-124 was performed by multiplex methylation specific real-time PCR.Results Positive methylation status was detected in 41 cases (58.6%). Number of blocks with HSIL varied from 1 to 13. Glandular extension was seen in 44 cases with number of blocks involved ranging from 1 to 10. The depth of HSIL glandular extension varied.Conclusion Methylation status in cervical HSIL does not correlate with the size of the lesion (measured by the number of blocks involved) or with HSIL propensity for endocervical glandular extension, nor with HPV type or multi-infection.Disclosure The study was partially supported with grant provided by the Ministry of Health of the Czech Republic - Conceptual Development of Research Organization (Faculty Hospital in Pilsen - FNPl, 00669806). There are no any other financial disclosures from the authors.