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Tumoral programmed cell death 1 (PD1) expression in endometrial carcinoma is a prognostic marker for patient outcome
  1. Barin Feroz1,
  2. Teresa L Pan1,
  3. Katharina Leitner1,
  4. Christoph Ebner1,
  5. Katharina Steger1,
  6. Wanja Kildal2,
  7. Gunnar Kristensen2,
  8. Alain Gustave Zeimet1,
  9. Hubert Hackl3,
  10. Heidi Fiegl4,
  11. Christian Marth1 and
  12. Verena Wieser1
    1. 1Department of Obstetrics and Gynecology, Medical University of Innsbruck, Innsbruck, Austria
    2. 2Institute for Cancer Genetics and Informatics, Oslo University Hospital, Oslo, Norway
    3. 3Biocenter, Institute of Bioinformatics, Medical University of Innsbruck, Innsbruck, Austria
    4. 4Laboratory for Clinical Biochemistry, Department of Gynecology and Obstetrics, Medical University of Innsbruck, Innsbruck, Austria
    1. Correspondence to Dr Verena Wieser, Department of Obstetrics and Gynecology, Medical University of Innsbruck, Innsbruck, A-6020, Austria; verena.wieser{at}


    Objective Immune checkpoint inhibitors have recently demonstrated benefit in patients with advanced and recurrent endometrial carcinoma. This retrospective study investigated immune checkpoint molecules in endometrial carcinoma as they pertain to the molecular subtypes, clinical outcomes, and predictive value.

    Methods Tumoral RNA expression of genes controlling the immune checkpoint, programmed cell death 1 (PD1, encoded by PDCD1), its ligand (PDL1, encoded by CD274), and interferon gamma (IFNG) was determined in 239 endometrial carcinoma tissues by quantitative polymerase chain reaction (qPCR) and compared with endometrial tissue from 25 controls. A total of 81 endometrial carcinoma tissues were analyzed using the ProMiSe molecular classification, and patient trajectories were analyzed for the entire cohort. Findings were validated in an independent cohort from The Cancer Genome Atlas (TCGA; n=548).

    Results PD1, PDL1, and IFNG expression was significantly higher in endometrial carcinoma when compared with non-malignant control tissue with a mean expression of 0.12, 0.05, and 0.05 in control tissue and 0.44, 0.31, and 0.35 in endometrial carcinoma, respectively. POLE-mutated and mismatch repair-deficient (MMRd) (immunologically hot) tumors showed the highest expression of PD1 and IFNG. Increased expression of PD1, PDL1, and IFNG was associated with improved recurrence-free (HR 0.32, p<0.001; HR 0.30, p<0.001; HR 0.47, p=0.012, respectively), disease-specific (HR 0.38, p<0.001; HR 0.29, p<0.001; HR 0.45, p=0.017, respectively), and overall survival (HR 0.56, p=0.003; HR 0.38, p<0.001; HR 0.58, p=0.006, respectively). Cox regression confirmed the prognostic significance of PD1 for recurrence-free survival (HR 0.39, p=0.009) and PDL1 for overall survival (HR 0.55, p=0.037). The prognostic value of tumoral PD1 on recurrence-free survival, disease-specific survival, and overall survival was confirmed in the TCGA cohort.

    Conclusions Tumoral gene expression controlling the PD1 immune checkpoint, particularly expressed in “hot tumors”, predicted recurrence-free, disease-specific, and overall survival in patients with endometrial carcinoma in two independent cohorts. Evaluation of these genes could be used to stratify patients who qualify for immune checkpoint inhibitors, which warrants prospective clinical trials.

    • Gynecology
    • Uterine Cancer
    • Endometrial Neoplasms

    Data availability statement

    Data are available in a public, open access repository. Data are available upon reasonable request.

    This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, an indication of whether changes were made, and the use is non-commercial. See:

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    • Blocking immune checkpoints such as programmed cell death 1 (PD1) or its ligand PDL1 is a potential treatment option for patients with advanced endometrial carcinoma. While patients with mismatch repair-deficient (MMRd) tumors usually benefit from immune checkpoint inhibitor treatment, other predictors are not well understood.


    • Our study reveals that immune checkpoint molecules can serve as prognostic markers in endometrial carcinoma, particularly tumoral PD1, which predicts clinical outcomes of these patients. Furthermore, PD1 is upregulated in immunologically hot tumors, which are known to respond well to immune checkpoint blockade.


    • PD1 expression could stratify patients for immune checkpoint inhibitor therapy in endometrial carcinoma; however, prospective clinical trials are needed to confirm this concept.


    Endometrial carcinoma is the most common gynecological tumor in Europe, with increasing incidence worldwide that may partly be explained by accumulating risk factors such as aging and obesity.1 The prognosis for patients diagnosed with early-stage endometrial carcinoma remains favorable, whereas recurrent or metastatic disease is associated with poor outcome due to limited surgical and systemic (targeted) treatment options.2 In recent years, introduction of molecular groups has allowed for implementation of precision medicine in endometrial carcinoma.3 Such molecular classification categorizes four distinct molecular groups based on their transcriptional profile4 5: DNA polymerase epsilon (POLE)-ultramutated (POLEmut, ie, POLE EDM), mismatch-repair deficient (MMRd (ie, microsatellite instable (MSI)), no specific molecular profile (NSMP; ie, p53-wt), and p53 aberrant (ie, p53-abn, p53-mut). Observing that patients with POLEmut endometrial carcinoma exhibit the best outcome and patients with p53-abn endometrial carcinoma have the poorest clinical survival has clarified the prognostic value of these parameters and may guide therapeutic decisions.6

    Immune checkpoint inhibitors blocking CTLA-4 or programmed death 1 (PD-1) or its ligand PD-L1 have demonstrated robust therapeutic efficacy in various cancer entities even in advanced stages, and have revolutionized the practice of medical oncology.7 The PD-1 axis is an immunosuppressive pathway that allows tumor cells to remain undetected by the immune system. In more detail, interferon-γ (IFN-γ) is common in the tumor microenvironment and body inflammation and induces the transcription of the PDL1 gene, which encodes for the programmed death-ligand 1 (PD-L1).8 When engaged to its receptor, PD-1 strongly interferes with T-cell receptor signal transduction allowing the tumor cell to escape immune-induced apoptosis. Interfering with PD-1 signal transduction either by antibody blockade or any other means enhances T-cell functions by potentiating signal transduction from the T-cell receptor (TCR) signalosome and inducing programmed cell death.9 However, not every tumor entity responds to immune checkpoint inhibitors and efficacy varies between tumor types and patients. To improve success of therapy (and diminish potentially unnecessary toxicity of these compounds) predictive markers of response to immune checkpoint inhibition reflect an unmet clinical need for most tumor types.10

    In 2017, pembrolizumab was approved by the US Food and Drug Administration (FDA) for patients with mismatch repair deficient (MMRd) or high microsatellite instable (MSI-H) tumors.2 11 Two years later, the FDA provided breakthrough therapy designation to lenvatinib combined with pembrolizumab for the treatment of patients with advanced endometrial carcinoma that has progressed after at least one previous systemic therapy.2 12 Dostarlimab, a PD-1 inhibitor, has recently also been approved for patients with advanced MSI-H/MMRd endometrial carcinoma.3 13 14 Ongoing studies are investigating immune checkpoint inhibition combined with other targeted agents such as poly (ADP-ribose) polymerase inhibitors (PARPis).15 In endometrial carcinoma, MSI-H or MMRd are predictors for immune checkpoint inhibitors; however, predictors for other genomic endometrial carcinoma subtypes are warranted.2

    We hypothesized that the expression profile of genes involved in immunosuppressive pathways are of prognostic value in endometrial carcinoma. Here, we have investigated the expression of the immune checkpoint genes PD1 and PDL1 and their regulator IFNG in 239 endometrial carcinoma patients. We further analyzed the association with clinicopathological features and molecular subtypes and validated their predictive value in a second independent cohort comprising 548 patients (The Cancer Genome Atlas (TCGA) dataset).


    Patients and Samples

    Endometrial tissue specimens from 239 endometrial carcinoma patients obtained at primary surgery and control tissue from 25 patients undergoing hysterectomy for non-malignant conditions such as fibroids were collected and processed by the Department of Obstetrics and Gynecology of the Medical University of Innsbruck between 1989 and 2015 as described recently.16

    The Ethics Committee of the Medical University of Innsbruck (Ref. No.: 1210/2021) approved the study, which was conducted in accordance with the Declaration of Helsinki. Bokhman’s type I and II classification was used to assess carcinoma risk. Patient characteristics are listed in Online Supplemental Table 1.

    Supplemental material

    RNA Isolation and Reverse Transcription

    Total cellular RNA extraction from endometrial tissue specimens and transcription were performed as described previously.16

    Quantitative Real-time Polymerase Chain Reaction (qPCR)

    Assays on demand for checkpoint genes PD1 (PDCD1; Hs01550088_m1), PDL1 (CD274; Hs00204257_m1), and interferon gamma (IFNG) (Hs00174143_m1) were purchased from Thermo Fisher Scientific (Waltham, MA, USA) as well as assays for the endogenous controls TATA box-binding protein (Hs99999910_m1)16.

    ProMiSe Molecular Subtypes

    In a cohort of 81 patients, molecular subtypes were defined and assigned according to the ProMisE criteria.17 Expression of MMR-proteins (MLH1, MSH2, MSH6, and PMS2) and p53 expression was assessed by immunohistochemistry. POLE mutation status was assessed by SNP mutation analysis for five known hotspots: P286R, V411L, S459F, S297F, and A456P. Patient characteristics are listed in Online Supplemental Table 2.

    TGCA Cohort

    To validate our results all analyses were performed on the TCGA publicly available dataset retrieved via and from previous TGCA analysis.18 Qualified patients were those with endometrial carcinoma and comprehensive data on age at diagnosis, tumor grade, International Federation of Gynecology and Obstetrics (FIGO) stage, survival, and gene expression analyses. Patient characteristics are listed in Online Supplemental Table 3.

    Statistical Analysis

    The non-parametric Mann–Whitney U test or Kruskal–Wallis test were applied to determine statistical significance between two or more groups, respectively. The Shapiro–Wilk test was used to test for normal distribution of data. For normal distributed data the Student’s two-tailed t-test was used to test for statistical significance between two groups. Correlations between PD1, PDL1, and IFNG mRNA expression were analyzed using Spearman’s rank correlation analysis. Univariate Kaplan–Meier analyses were conducted to explore the association of checkpoint gene expression with recurrence-free, disease-specific, and overall survival. The parameters that demonstrated an influence on the outcome in the univariate analysis (p<0.05) were subjected to multivariate Cox regression analyses.

    For survival analyses patients were divided into low and high mRNA expression level groups by the optimal cut-off expression value calculated by Youden’s index as previously described.16 We chose the Youden index because it integrates sensitivity and specificity for each transcript with a value that ranges from 0 to 1. The index measures the effectiveness of a diagnostic marker and at the same time proposes an optimal threshold (cut-off) for the biomarker of interest.19 We used the proposed cut-off to segregate groups into patients with “high” and “low” expression. Statistical analysis was performed using the SPSS statistical software (version 29.0.0; SPSS, Chicago, IL, USA).


    Immune Checkpoint Regulators are Overexpressed in Endometrial Carcinoma

    We investigated PD1, PDL1, and IFNG expression in endometrial carcinoma patients and compared the expression to that of non-malignant control endometrial tissue by qPCR as previously described for ovarian cancer.20 Notably, the expression of PD1, PDL1, and its inducer IFNG were increasingly expressed in endometrial carcinoma when compared with non-malignant endometrial tissue (Figure 1). More specifically, PD1 expression was seven-fold (p<0.001; Figure 1A), PDL1 expression was three-fold (p<0.001; Figure 1B), and IFNG expression was five-fold (p<0.001; Figure 1C) increased when compared with control tissue. Next, we sought to define the impact of clinical subtypes of endometrial carcinoma to the expression of PD1, PDL1, and IFNG. Most notably, PD1 and PDL1 were increasingly expressed in low FIGO stages (FIGO Stages I and II) compared with advanced stages (p=0.007 and p=0.003, respectively; Online Supplemental Figure 1A,B). Furthermore, PD1 expression was increased in younger patients (≤68.8 years; p=0.043) as demonstrated in Online Supplemental Figure 1C.

    Figure 1

    Programmed cell death 1 (PD1), its ligand (PDL1), and interferon gamma (IFNG) are increasingly expressed in endometrial carcinoma. Transcriptional levels of PD1 (A), PDL1 (B), and IFNG (C) in endometrial carcinoma (EC, n=239) compared with non-malignant control tissue (n=25). mRNA expression was normalized to TATA box-binding protein (TBP).

    The highest levels of IFNG were detected in high-grade endometrial carcinoma (p=0.010), which was the most frequent histological subtype in our cohort (Online Supplemental Figure 1D). Furthermore, we observed a strong correlation between the expression of PD1, PDL1, and IFNG in endometrial carcinoma tissue (Online Supplemental Table 4). In more detail, PD1 correlated with PDL1 (p<0.001, rS=0.685), IFNG with PD1 expression (p<0.001, rS=0.804), and IFNG with PDL1 (p<0.001, rS=0.718) expression.

    High Expression of Immune Checkpoints is Associated with Improved Survival

    To evaluate the impact of intratumoral checkpoint molecule expression on clinical outcome we followed 239 patients for a median observation period of 5.8 years. Applying the Youden index to define a cut-off for PD1, PDL1, and IFNG transcripts, patients were stratified into a group with high expression of each transcript and a group with low expression, respectively. The univariate survival analysis revealed that high intratumoral expression of PD1, PDL1, and IFNG was associated with better outcome (Online Supplemental Table 5). As demonstrated in Figure 2, high expression of PD1, PDL1, and IFNG was associated with better recurrence-free survival (Figure 2A–C) (HR 0.32, 95% CI 0.19 to 0.53, p<0.001; HR 0.30, 95% CI 0.17 to 0.52, p<0.001; HR 0.47, 95% CI 0.26 to 0.86, p=0.012, respectively), disease-specific survival (Figure 2D–F) (HR 0.38, 95% CI 0.22 to 0.67, p<0.001; HR 0.29, 95% CI 0.15 to 0.55, p<0.001; HR 0.45, 95% CI 0.23 to 0.88, p=0.017, respectively), and overall survival (Figure 2G–I) (HR 0.56, 95% CI 0.38 to 0.83, p=0.003; HR 0.38, 95% CI 0.24 to 0.62, p<0.001; HR 0.58, 95% CI 0.39 to 0.86, p=0.006, respectively).

    Figure 2

    High expression of programmed cell death 1 (PD1), its ligand (PDL1), and interferon gamma (IFNG) is associated with improved clinical outcome in endometrial carcinoma (n=239). PD1 mRNA expression in endometrial carcinoma patients and (A) recurrence-free survival, (D) disease-specific survival, and (G) overall survival. PDL1 mRNA expression and (B) recurrence-free survival, (E) disease-specific survival, and (H) overall survival. IFNG mRNA expression and (C) recurrence-free survival, (F) disease-specific survival, and (I) overall survival. mRNA expression was normalized to TATA box-binding protein (TBP). CI, confidence interval; HR, hazard ratio.

    Considering the long study period (ranging from 1989 to 2015), we next assessed whether adjuvant therapy posed a potential bias. In our cohort, 49 of 239 patients received adjuvant chemotherapy (22.1%) and 206 patients received adjuvant radiation therapy (86.2%). In more detail, 45 patients received adjuvant polychemotherapy, 4 received adjuvant platinum monotherapy, 131 received vaginal brachytherapy, and 75 received brachytherapy combined with external body radiation therapy. Notably, subgroups analysis on recurrence-free survival confirmed the prognostic value of PD1 in patients with polychemotherapy (HR 0.28, 95% CI 0.11 to 0.72, p=0.005; Online Supplemental Figure 2A) and without chemotherapy (HR 0.30, 95% CI 0.17 to 0.55, p<0.001; Online supplemental figure 2B). A similar trend was demonstrable in patients receiving vaginal brachytherapy (HR 0.49, 95% CI 0.17 to 1.42, p=0.181, Online Supplemental Figure 2C) or brachytherapy combined with external body radiation therapy (HR 0.15, 95% CI 0.23 to 1.26, p=0.15, Online Supplemental Figure 2D); however, this did not reach statistical significance. As such, subgroup analyses suggested that adjuvant therapy is unlikely to be a confounder in our study.

    PD1 and PDL1 Predict Endometrial Carcinoma Survival

    By performing a multivariate analysis (Table 1) we identified that high FIGO stages, tumor grade 3, and age >68.8 years were evaluated as independent factors negatively predicting clinical outcome in our cohort. Notably, also high expression of PD1 and PDL1 were identified as independent prognostic factors for clinical outcome: High expression of PD1 was predictive for recurrence-free survival (HR 0.39, 95% CI 0.19 to 7.93, p=0.009) in our cohort, while high PDL1 expression was predictive for overall survival (HR 0.55, 95% CI 0.32 to 0.97, p=0.037).

    Table 1

    Multivariable Cox regression analysis in the Innsbruck cohort (n=239)

    Validation of the Prognostic Value in the TCGA Cohort

    To validate these findings in an independent cohort, we applied the previous cut-offs of PD1, PDL1, and IFNG expression to the TCGA dataset (n=548; demographic features shown in Online Supplemental Table 3). As similarly observed in our cohort, high PD1 expression (but not PDL1 expression) was associated with improved clinical outcome (recurrence-free and overall survival p<0.001 and disease-specific survival p=0.004; Online Supplemental Table 6 and Online Supplemental Figure 3). By performing a multivariate analysis in the TCGA cohort, PD1 was identified to be predictive for recurrence-free survival (HR 0.55, 95% CI 0.39 to 0.78, p<0.001), disease-specific survival (HR 0.51, 95% CI 0.30 to 0.87, p<0.012), and overall survival (HR 0.49, 95% CI 0.32 to 0.75, p<0.001), underlining the value of our inception cohort (Table 2).

    Table 2

    Multivariable Cox regression analysis in The Cancer Genome Atlas (TCGA) cohort (n=548)

    PD1 and IFNG are Elevated in “Hot Tumors”

    To evaluate the expression of checkpoint molecules and IFNG in the four prognostically distinguishable molecular subtypes, that is, POLEmut, MMRd, NSMP, and p53-mut, 81 samples of our cohort were analyzed according to the ProMisE classification. ProMisE molecular classification yielded 35 (43.2%) MMRd, 8 (9.9%) POLEmut, 32 (39.5%) NSMP, and 6 (7.4%) p53-mut. Applying this classification, we found an almost three-fold (2.9) induction of PD1 (p=0.019) and a five-fold (5.3) induction of IFNG (p<0.001) in POLEmut endometrial carcinoma compared with other subgroups (Online Supplemental Figure 4). We further divided molecular subtypes into immunologically “hot tumors” (POLEmut and MMRd) and immunologically “cold tumors” (NSMP and p53-mut). Notably, “hot tumors” showed higher expression of PD1 (p=0.015; Figure 3A) and IFNG (p<0.001; Figure 3B) compared with “cold tumors”.

    Figure 3

    Programmed cell death 1 (PD1) (A) and interferon gamma (IFNG) (B) are highly expressed in immunologically “hot tumors” (n=43) compared with “cold tumors” (n=38). mRNA expression was normalized to TATA box-binding protein (TBP). “Hot tumors” comprise POLE-mutated and mismatch repair-deficient (MMRd) molecular subtypes (HOT) and “cold tumors” comprise no specific molecular profile (NSMP) and p53-mut molecular subtypes (COLD).


    Summary of Main Results

    We investigated PD1, PDL1, and IFNG (as regulator of PDL1) in endometrial carcinoma. Tumor tissue depicted increased expression of these genes when compared with non-malignant control tissue. More importantly, high expression of PD1 and PDL1 was associated with improved clinical outcome, (longer recurrence-free, disease-specific, and overall survival). Notably, PD1 is prognostic for recurrence-free, disease-specific, and overall survival independent of other clinicopathological characteristics such as age, FIGO stage, or tumor grading. Furthermore, PD1 expression was associated with better clinical outcome in an independent validation cohort.

    Results in the Context of Published Literature

    Previous data on the association of checkpoint molecules with clinical outcome in endometrial carcinoma are conflicting. Yamashita et al demonstrated that immunohistochemically high PD-L1 is associated with better recurrence-free survival; however, there was no association with overall survival or PD-1 and endometrial carcinoma outcome, respectively.21 Zong et al showed that PD-L1 positivity in tumor cells is associated with a favorable prognosis in patients with high-risk endometrial carcinoma.22 High expression of stromal PD-1 in early endometrial carcinoma was demonstrated to reduce risk of relapse.23 Other studies did not observe any associations of these checkpoint molecules with survival.24–26

    A recent meta-analysis including four studies providing information on immunohistochemical PD-L1 expression and overall survival21 26 27 indicated that PD-L1 overexpression had a non-significant association with overall survival.28 Our results are in line with the data of Mendiola et al23 and strengthen the prognostic significance of checkpoint molecules, especially of PD1, in endometrial carcinoma. Nonetheless, further studies with large sample sizes are needed to clarify the clinical utility of these biomarkers.

    Controversial results regarding PD-1 or PD-L1 expression and patients’ outcome extend to other tumor entities like lung and colorectal cancer or melanoma, which can be explained by different antibodies used for immunohistochemistry quantification, different cut-off values, and observer interpretation of staining positivity and heterogeneous expression in the tumor.29

    Strengths and Weaknesses

    In contrast to previous studies on endometrial carcinoma, which used immunohistochemistry for the quantification of PD-1 and PD-L1, our findings are based on mRNA expression. By doing so, we may avoid the abovementioned concerns about immunohistochemistry quantification, namely interobserver disagreement. Furthermore, this may also compensate intra-tumor heterogeneity, which can only poorly be controlled in immunohistochemistry. The significance of our results can be depicted by the validation in the TCGA cohort. TCGA sample collection was performed in a similar fashion as in our discovery cohort (eg, primary, untreated tumor), and our discovery cohort (Innsbruck) and validation cohort (TCGA) demonstrated similar clinicopathological patient characteristics (Online Supplemental Table 7), thus supporting the validity of our validation approach.

    In our study, transcriptional analysis differs between these cohorts, which reflects a limitation of our study. More specifically, RNA expression was analyzed by qPCR in the discovery cohort (Innsbruck), while expression in the TCGA was analyzed by RNA sequencing. Publicly available data on the human protein atlas providing a dataset of 548 patients with endometrial carcinoma also demonstrate a favorable outcome for patients with high intra-tumor PD1 RNA expression (data not shown). Another limitation of our study is its reliance on retrospective data; prospective data and larger cohort sizes (considering the broad 95% confidence intervals of PD1 and PDL1 as prognostic markers) are needed to validate PD1 and PDL1 expression as predictive markers for immune checkpoint inhibitor treatment response and patient outcome in endometrial carcinoma. Tumoral PD1 serves as a robust prognostic marker for recurrence-free, disease-specific, and overall survival, which we confirmed in an independent TCGA cohort. By contrast, the prognostic value of tumoral PDL1 expression on clinical outcome appeared less consistent in our study. More specifically, increased PDL1 expression was associated with improved recurrence-free, disease-specific, and overall survival in the univariate survival analyses, while the prognostic value was not confirmed by Cox regression in both study cohorts. A subgroup analysis by FIGO stage, histology, age, or ProMisE classification did not reveal a more consistent prognostic value of PDL1 (data not shown). As such, our approach could not confirm PDL1 as a reliable biomarker, which has been similarly described for other tumor entities.30

    Implications for Practice and Future Research

    Patients with MMRd tumors exhibit response rates that top response rates of other molecular endometrial carcinoma subtypes.11 It is well established that MMRd tumors are immunologically “hot” due to high tumor mutational burden and consequent increased lymphocyte infiltration, a feature of immune response.11 Therefore, blocking inhibitory signals such as PD-1 and PD-L1 in the tumor microenvironment in “hot tumors” enables the immune system to fight cancer, thereby achieving long-term response rates. Investigating expression of checkpoints in molecular subtypes according to ProMisE molecular classifiers5 we found the highest levels of PD1, PDL1, and IFNG in POLEmut and MMRd tumors. This is in line with previous results, namely that PD-L1 expression was more frequent in POLEmut and MMRd subtypes than in p53-mutant and NSMP subtypes.22 POLEmut and MMRd endometrial carcinomas are associated with high neoantigen loads and number of TILs, which is counterbalanced by overexpression of PD-1 and PD-L1.31 Based on our results we hypothesize that patients with high intra-tumor PD1 expression, which was especially found in POLEmut and MMRd endometrial carcinoma, may demonstrate remarkable response rates to immune checkpoint inhibitors. While it has already been clearly established that immune checkpoint inhibitor is greatly effective in patients with MMRd tumors,11 emerging clinical evidence indicates that POLEmut tumors (ie, cases of colorectal and endometrial cancers) may also respond extraordinarily well to immune checkpoint inhibitors.32 33


    Our data demonstrate that expression of tumoral immune checkpoint transcripts, especially PD1, predicts clinical outcome in endometrial carcinoma. PD1 is upregulated in immunologically hot tumors, which are known to demonstrate good response rates to immune checkpoint blockade. Therefore, PD1 expression could be used to stratify patients qualifying for immune checkpoint inhibitor therapy in endometrial carcinoma, but considering the retrospective nature of our findings, establishing this concept warrants controlled prospective clinical trials.

    Data availability statement

    Data are available in a public, open access repository. Data are available upon reasonable request.

    Ethics statements

    Patient consent for publication

    Ethics approval

    This study involves human participants and was approved by the Ethics Committee of the Medical University of Innsbruck (Ref. No.: 1210/2021). Participants gave informed consent to participate in the study before taking part.


    We thank Kathrin Außerlechner and Brigitte Greiderer-Kleinlercher for their excellent technical support.



    • BF and TLP contributed equally.

    • Contributors Study concept and design: BF, TLP, CM, VW. Provision of materials or patients: BF, TLP, HF, CE, HH. Analysis and interpretation of data: BF, TLP, GK, WK, VW. Manuscript writing: BF, TLP, VW. Critical review of the manuscript: all authors contributed. Final approval of manuscript: all authors contributed. Guarantor: VW.

    • Funding The project was supported by the Tiroler Wissenschaftsförderung (TWF; grant number F.16950; to VW) and Medizinischer Forschungsfonds Tirol (MFF; grant number 339; to VW), the National Bank of Austria (OeNB) (grant number 18279; to HH), and the Verein zur Krebsforschung in der Frauenheilkunde, an association that is exclusively financed by donation funds for cancer research in female malignancies.

    • Competing interests BF reports travel expenses from Roche, Pfizer, and Lilly. TLP reports travel expenses from MEDahead. CE reports travel expenses from GSK, PharmaMar, Pfizer, AstraZeneca, and Lilly. KL reports support for attending meeting and travel from Gilead, GSK, Eisai, and Roche. HF reports no conflicts of interest. GK reports no conflicts of interest. WK reports no conflicts of interest. KS reports travel expenses from Roche, Daiichi Sankyo, GSK, and Pharma Mar. AGZ reports consulting fees from Amgen, Astra Zeneca, GSK, MSD, Novartis, PharmaMar, Roche-Diagnostics, and Seagen; honoraria from Amgen, Astra Zeneca, GSK, MSD, Novartis, PharmaMar, Roche, and Seagen; travel expenses from Astra Zeneca, Gilead, and Roche; participation on advisory boards from Amgen, Astra Zeneca, GSK, MSD, Novartis, Pfizer, PharmaMar, Roche, and Seagen. HH reports research funding from CatalYm and Secarna. VW reports honoraria from Roche and Novartis; travel expenses from Roche; participation on advisory boards from Novartis. CM reports consulting fees from Roche, Novartis, Amgen, MSD, PharmaMar, Astra Zeneca, GSK, and Seagen; honoraria from Roche, Novartis, Amgen, MSD, PharmaMar, Astra Zeneca, GSK, and Seagen; travel expenses from Roche and Astra Zeneca; participation on advisory boards from Roche, Novartis, Amgen, MSD, Astra Zeneca, Pfizer, PharmaMar, GSK, and Seagen. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.

    • Provenance and peer review Not commissioned; externally peer reviewed.

    • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.