Background Minimally invasive surgery in cervical cancer has demonstrated in recent publications worse outcomes than open surgery. The primary objective of the SUCCOR study, a European, multicenter, retrospective, observational cohort study was to evaluate disease-free survival in patients with stage IB1 (FIGO 2009) cervical cancer undergoing open vs minimally invasive radical hysterectomy. As a secondary objective, we aimed to investigate the association between protective surgical maneuvers and the risk of relapse.
Methods We obtained data from 1272 patients that underwent a radical hysterectomy by open or minimally invasive surgery for stage IB1 cervical cancer (FIGO 2009) from January 2013 to December 2014. After applying all the inclusion-exclusion criteria, we used an inverse probability weighting to construct a weighted cohort of 693 patients to compare outcomes (minimally invasive surgery vs open). The first endpoint compared disease-free survival at 4.5 years in both groups. Secondary endpoints compared overall survival among groups and the impact of the use of a uterine manipulator and protective closure of the colpotomy over the tumor in the minimally invasive surgery group.
Results Mean age was 48.3 years (range; 23–83) while the mean BMI was 25.7 kg/m2 (range; 15–49). The risk of recurrence for patients who underwent minimally invasive surgery was twice as high as that in the open surgery group (HR, 2.07; 95% CI, 1.35 to 3.15; P=0.001). Similarly, the risk of death was 2.42-times higher than in the open surgery group (HR, 2.45; 95% CI, 1.30 to 4.60, P=0.005). Patients that underwent minimally invasive surgery using a uterine manipulator had a 2.76-times higher hazard of relapse (HR, 2.76; 95% CI, 1.75 to 4.33; P<0.001) and those without the use of a uterine manipulator had similar disease-free-survival to the open surgery group (HR, 1.58; 95% CI, 0.79 to 3.15; P=0.20). Moreover, patients that underwent minimally invasive surgery with protective vaginal closure had similar rates of relapse to those who underwent open surgery (HR, 0.63; 95% CI, 0.15 to 2.59; P<0.52).
Conclusions Minimally invasive surgery in cervical cancer increased the risk of relapse and death compared with open surgery. In this study, avoiding the uterine manipulator and using maneuvers to avoid tumor spread at the time of colpotomy in minimally invasive surgery was associated with similar outcomes to open surgery. Further prospective studies are warranted.
- cervix uteri
- surgical oncology
- neoplasm recurrence, local
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Minimally invasive surgery in patients with FIGO 2009 stage IB1 cervical cancer increased the risk of relapse and death.
Patients that underwent minimally invasive surgery using a uterine manipulator had a 2.76-times higher hazard of relapse.
Avoiding uterine manipulator and implementing a protective vaginal closure was associated with disease-free-survival similar to that of open surgery.
On November 16, 1898, in Vienna, Dr. Ernst Wertheim – who was 34 years' old at the time – performed the first radical abdominal hysterectomy for cervical cancer in Europe.1 Since then, early cervical cancer has been successfully treated by radical hysterectomy over the past 120 years. Initially, by open or vaginal approach, and more recently (since 1991), by minimally invasive surgery,2 3 either by laparoscopy or robotic surgery. For 25 years, multiple retrospective publications reported the feasibility, advantages, and oncologic safety of this minimally invasive approach.4–7 However, two recent manuscripts published in October of 2018 in NEJM (the LACC trial and a large epidemiologic study involving women from Cancer-Accredited hospitals in the United States)8 9 demonstrated higher rates of recurrence and death in patients that underwent minimally invasive radical hysterectomy. Subsequently, a number of retrospective studies corroborated these findings,10–15 resulting in a recent modification of the NCCN, European Society of Gynecological Oncology (ESGO), and ESMO guidelines.16–18
The unexpected results of the LACC trial and other retrospective studies led to an ongoing discussion regarding the ideal surgical approach in patients with early cervical cancer. Also, the potential of implementing new trials containing a minimally invasive approach arm has been difficult, given the challenges of offering an option of treatment that has been deemed oncologically inferior. Moreover, the reasons for the inferior outcomes of minimally invasive surgery have not yet been elucidated.19
In 2019, the ESGO conducted a survey named “after LACC trial”, attempting to understand the perspective of their members on this topic. The study showed that 83% of respondents did not expect these results, and 4 months after the publication of the LACC trial, 56% had changed their surgical approach from minimally invasive surgery to open radical hysterectomy.20 In Europe, there was a lack of updated information regarding the outcomes on the surgical approach of radical hysterectomy. With this in mind, we designed the SUCCOR (Surgery in Cervical Cancer, Observational, Retrospective) study, comparing minimally invasive vs open abdominal radical hysterectomy in patients with FIGO 2009 stage IB1 cervical cancer. The goal of this study was to determine disease-free survival of radical hysterectomy between the two surgical approaches in Europe. Second, we aimed to explore the risk of relapse between the two approaches, with a particular focus on the surgical maneuvers (use of uterine manipulator, protective closure of colpotomy over the tumor). Lastly, we also wished to analyze the influence of the aforementioned variables based on tumor size.
Study design and endpoints
The study was a European, multicenter, observational, retrospective, cohort study spanning from January 1, 2013 to December 31, 2014 with the primary goal of comparing disease-free survival at 4.5 years in patients who underwent a laparoscopic or robotic radical hysterectomy vs abdominal hysterectomy for FIGO 2009 stage IB1 cervical cancer. The secondary endpoints included a comparison of overall survival at 4.5 years between groups and to explore the association between specific surgical maneuvers and the risk of relapse. More specifically, we aimed to determine if the use of a uterine manipulator and protective vaginal closure over the cervix to avoid tumor spread at the time of the colpotomy might impact the outcome of patients undergoing minimally invasive surgery. Lastly, we investigated the influence of tumor diameter on oncologic outcomes. Disease-free survival was defined as the time from the date of the radical hysterectomy to time of relapse or last contact. Overall survival was calculated from the date of the radical hysterectomy to the time of last contact or death from cervical cancer. We inquired on the use of the uterine manipulator given previously published data suggesting its use as an etiology for the worse outcomes in minimally invasive surgery.21 22 The protective vaginal closure over the tumor has been advocated to avoid contact between the cervical tumor and the abdominal cavity after the colpotomy.23
We registered the study at ClinicalTrials.gov with the Identifier NCT03958305.24 A copy of the protocol is provided in the online supplementary appendix. All authors contributed meaningfully to the conception or design of the work or the acquisition, analysis, or interpretation of data for the study. The authors confirm the completeness and accuracy of the data and analyzes, the fidelity of the study to the protocol, and the final approval of the version to be published.
Inclusion and exclusion criteria
We designed a list of inclusion-exclusion criteria to define a homogeneous population. Patients were eligible if they underwent a radical hysterectomy in a European Institution for stage IB1 cervical cancer (FIGO 2009), from January 1, 2013 to December 31, 2014. Inclusion criteria included patients age 18 years' old or older and squamous cell carcinoma, adenocarcinoma, or adenosquamous carcinoma. A pelvic MRI confirming a tumor diameter less or equal than 4 cm with no parametrial invasion and a preoperative CT scan, MRI, or PET-CT without extra-cervical metastatic disease, were mandatory. The operative report had to describe either a Type B–C radical hysterectomy by minimally invasive surgery (laparoscopic or robotic) or by open surgery with a bilateral pelvic lymphadenectomy, including at least a total of 10 pelvic nodes. Those who only underwent sentinel lymph node mapping were allowed in the study. There had to be documentation of tumor size, margins, and nodal status.
Patients were excluded when any other histological type was diagnosed; if tumor size was larger than 4 cm; and when there was a past history of any invasive tumor, previous chemotherapy or radiation, suspicious lymph nodes, or metastatic disease on preoperative imaging. Conversion from minimally invasive surgery to laparotomy was cause for exclusion. Finally, patients with cervical conization prior to radical hysterectomy were excluded from the primary endpoint analysis. Reasons for not including patients with the previous conization were as follows: cone biopsies were often performed at outside institutions thus leading to missing critical pathological information; insufficient pathology report to stage the tumor adequately; lack of consensus on measurement of tumor size when cone biopsy and final specimen both had residual disease; consideration that conization might be a source of bias given that, if no residual tumor, then risk for tumor dissemination may be lower than that of patients with gross tumor; and lastly, we are currently working on an additional manuscript that focuses on this patient group within the SUCCOR database.
Accrual and data source
We invited all ESGO members to participate in this study. Researchers from 126 institutions in 29 European countries registered and contributed to the project. After obtaining ethical consent from our central IRB, we required a Certificate of Approval or a Letter of Exemption by the local Ethical Committees from all the investigators. An anonymized complete Case Record Form, including 123 items (online supplementary file 1), was sent to all the investigators. After completing the case collection, all researchers signed a final declaration affirming that all the submitted data matched the data in the patients' charts. As far as the researcher was able to analyze, data included all cases operated on at the respective institutions.
In our primary analyzes when comparing open vs minimally invasive surgical approach, we hypothesized 10% of relapse in the group that underwent open surgery. Assuming a two-sided alfa error of 5% and an 80% of statistical power, 261 women were needed in each group to detect a difference between groups of 9% in the risk of relapse. We used inverse probability of treatment weighting based on propensity score to construct a weighted cohort of patients who differed with respect to surgical approach but were similar with respect to other measured characteristics.25 To calculate the inverse probability of treatment weights, each patient’s propensity to undergo open hysterectomy was estimated using a logistic regression model that included predictor variables that had been selected based on their a priori possibility of confounding the relationship between surgical approach and survival (largest tumor diameter, tumor grade, lymphovascular invasion, depth of invasion, margins status, nodal status, and adjuvant therapy).26 We assigned patients who underwent open surgery a weight of 1/(propensity score) and those who underwent minimally invasive surgery a weight of 1/ (1 − propensity score). To reduce the variability in the inverse probability of treatment-weighted models, we used stabilized weights in all the analyzes. To explore whether the observed associations differed according to largest tumor size, we performed a subgroup analysis.
In the secondary analyzes, we calculated the inverse probability of treatment weights considering open surgery, minimally invasive surgery without uterine manipulator, and minimally invasive surgery with a uterine manipulator. We used a multinomial logistic regression model with the same predictors in the primary analysis. We obtained three propensity scores and assigned each patient weight of 1/(the correspondent propensity score). Finally, we followed the same calculations considering open surgery and minimally invasive surgery with and without vaginal protective closure. We applied the same multinomial logistic regression model with the identical predictors in the principal analysis. Besides, we obtained three propensity scores as in the evaluation of the uterine manipulator use.
Participants with missing values in quantitative variables were excluded from the analyzes. Missing values in qualitative variables were classified into a new category. Distributions of categorical variables were compared using the chi-square test in the unweighted cohort and weighted logistic-regression models in the weighted cohort. Quantitative variables were compared using the student t-test in the unweighted cohort and weighted linear-regression models in the weighted cohort.
We compared disease-free survival and overall survival using the inverse probability of treatment-weighted log-rank test and plotted weighted incidence functions using Nelson–Aalen survival curves. We estimated the hazard ratio (and 95% CI) for disease-free survival and overall survival after each of the different surgical approach using open surgery as the category of reference. After analyzing the proportionality of hazard with the specific test and assessing surgical approach was not a time varying covariate, we used weighted Cox proportional-hazards models in all the analyzes. The analyzes were performed with Stata 15.0. and SPSS v26.0. P-values below 0.05 were considered statistically significant.
From May 15 to November 15, 2019, we received data from a total of 1272 patients that underwent a radical hysterectomy in Europe for stage IB1 cervical cancer during 2013–2014 (Figure 1). A total of 156 patients did not meet the inclusion-exclusion criteria or had missing information on follow-up and were excluded: of the remaining 1116 patients, 37.9% (423 patients) had undergone a cone biopsy previous to radical hysterectomy and were excluded from the analysis. Data on 693 patients was analyzed for the primary endpoint. Of these patients, 291 underwent minimally invasive and 402 an open radical hysterectomy (see online supplementary appendix). Among patients in the minimally invasive surgery group, 228 (78.5%) underwent laparoscopic surgery and 63 (21.5%) robotics surgery. Patients in the minimally invasive surgery group had more favorable prognostic features. They were more likely to have smaller tumors, a lower percentage of positive nodes, and less frequently received adjuvant therapy. The final cohort was composed of 693 patients (open surgery, n=402; minimally invasive surgery group n=291) and, after weighting, did not show statistical differences between groups in the selected covariates (Table 1).
Survival analysis based on surgical approach
Patients in the weighted cohort had a median follow-up of 59 months (range; 1–83), 56 months in the minimally invasive surgery group, and 60 months in the open group. At 4.5 years of follow-up, there were 60 (20.6%) relapses in 291 patients in the minimally invasive surgery group while in the open surgery group there were 47 (11.7%) relapses in 402 patients. Disease-free survival at 4.5 years was 79% in the minimally invasive group and 89% in the open surgery group (P=0.0003). The risk of recurrence for patients who underwent minimally invasive surgery was twice that of the open surgery group (HR, 2.07; 95% CI, 1.35 to 3.15; P=0.001) (Figure 2A). We confirmed those differences in the subgroup of patients with tumors greater than 2 cm (HR, 2.31; ; 95% CI, 1.37 to 3.90; P=0.002) while in the group of patients with tumors smaller or equal than 2 cm those differences were not significant (HR, 1.63; 95% CI, 0.79 to 3.40; P=0.19)
Minimally invasive surgery was also associated with a lower rate of overall survival than open surgery. A total of 28 patients died in the minimally invasive surgery group while 21 patients died in the open surgery group. The risk of death was 2.42-times higher than in the open surgery group (HR, 2.42; 95% CI, 1.34 to 4.39; P=0.004). At 4.5 years, the proportion of living patients was 89% in the minimally invasive and 97% in the open surgery group (Figure 2B). Again, these differences were significant in patients with tumors greater than 2 cm (HR, 2.26; 95% CI, 1.18 to 4.36; P=0.014) but not in patients with tumors smaller or equal to 2 cm (HR, 2.77; 95% CI, 0.91 to 8.47; P=0.072)
Impact of uterine manipulator in minimally invasive surgery
A weighted cohort was built with the same covariates used in the surgical approach analysis, 652 patients (open, n=402; minimally invasive surgery without manipulator, n=106; and minimally invasive surgery with the manipulator, n=144). After the inverse probability of treatment weighting, survival analysis demonstrated a negative impact of uterine manipulators on disease-free survival. The manipulator group experienced 38 (26.3%) relapses in 144 patients compared with 17 (16%) relapses in 106 patients without a manipulator. The disease-free survival at 4.5 years was 73% in the uterine manipulator group and 83% in those without (P=0.0001). The patients that underwent minimally invasive surgery with a uterine manipulator had a 2.76-times higher chance of a relapse compared with those in the open approach (HR, 2.76; 95% CI, 1.75 to 4.33; P<0.001) (Figure 3A). The adverse effect of the uterine manipulator significantly impacted patients with tumors greater than 2 cm (HR, 3.05; 95% CI, 1.73 to 5.38; P<0.001) while tumors less or equal than 2 cm did not show differences (HR, 2.25; 95% CI, 0.96 to 5.26; P=0.06). Patients that underwent minimally invasive surgery without the uterine manipulator had similar rates of relapse to those who underwent open surgery (HR, 1.58; 95 % CI, 0.79 to 3.15; P=0.20). The use of a uterine manipulator adversely impacted overall survival in patients who underwent minimally invasive surgery (HR, 3.00; 95% CI, 1.60 to 5.63; P=0.001). In this case, the overall survival was significantly lower in all tumor sizes (tumors greater than 2 cm (HR, 2.69; 95% CI, 1.22 to 5.89; P=0.013) and tumors smaller or equal to 2 cm (HR, 3.84; 95% CI, 1.11 to 13.26; P=0.033) (Figure 3B) (See online supplementary file 1).
Protective vaginal closure in minimally invasive surgery
Lastly, we constructed a new weighted cohort to explore the impact of protective vaginal closure (Figure 4A). We used the same covariates used before (largest tumor diameter, tumor grade, lymphovascular invasion, depth of invasion, margins status, nodal status, and adjuvant therapy) to balance the three groups of patients: minimally invasive surgery patients with and without protective vaginal closure, and those with open surgery. Within this weighted cohort, 52 (25%) of 207 patients had a relapse among those without protective vaginal closure, while only 3 (7%) of 43 recurred amid those that underwent this protective surgical maneuver (P<0.001).
Disease-free survival at 4.5 years was 93% with protective vaginal closure and 74% in those without (P<0.001). Minimally invasive surgery patients without protective vaginal closure had a 2.58-times higher risk of recurrence than the open approach (HR, 2.58; 95% CI, 1.70 to 3.95; P<0.001). Moreover, those who underwent minimally invasive surgery with vaginal closure had similar rates of relapse to those who underwent open surgery (HR, 0.63; 95% CI, 0.15 to 2.59; P<0.52) (Figure 4B). These differences remained in patients with tumors >2 cm (HR, 2.99; 95% CI, 1.78 to 5.00; P<0.001). but were not found in those with tumors ≤2 cm (HR, 1.96; 95% CI, 0.91 to 4.27; P<0.09).
Minimally invasive surgery patients without vaginal closure had 2.85-times risk of death when compared with those that underwent an open approach (HR, 2.85; 95% CI, 1.59 to 5.15; P<0.001). We observed these differences in patients with tumors >2 cm (HR, 2.71; 95% CI, 1.35 to 5.46; P=0.005) and those with tumors ≤2 cm (HR, 3.33; 95% CI, 1.06 to 10.46; P=0.039).
Our study showed that in a European population, minimally invasive surgery increases the risk of relapse and death among patients with early cervical cancer. There was a 10% difference at 4.5-year disease-free survival between surgical approaches, which is consistent with the results of the LACC trial. This outcome particularly pertained to tumors >2 cm in diameter. However, one should note that the absence of statistical significance in small tumors does not translate to an absence of effect, given the lower incidence of events in this low-risk group. In addition, in this study, avoiding the use of uterine manipulator and a protective vaginal closure to prevent tumor spillage leads to oncologic outcomes that are similar to those of open surgery.
As it pertains to the findings on the uterine manipulator in the minimally invasive procedure, it should be highlighted that although it technically facilitates the procedure, it increases the risk of relapse by 2.76-times. Interestingly, patients that underwent a radical hysterectomy by minimally invasive surgery without the uterine manipulator had comparable outcomes to those with open surgery. This was noted in patients with tumors >2 cm but not in those with tumors ≤2 cm. In our study, patients that underwent minimally invasive surgery with protective vaginal closure showed similar rates of relapse to those who underwent open surgery.
Recently, two large nation-based retrospective studies, one from Denmark and the other from the Netherlands, failed to show differences in disease-free survival and overall survival comparing the open vs minimally invasive approach.27 28 The Danish study compares two periods before and after the introduction of robotic surgery from 2005 to 2017. Compared with SUCCOR, the groups were metachronous with a significantly different median follow-up in both groups (113.0 vs 42.4 months). In that study, the percentage of different surgical approaches in each study group is not clearly defined and conization was not considered a potential confounding factor. The Dutch study evaluates the outcome in a weighted cohort with a propensity matching score. Unlike our study, it did not include adjuvant therapy, margin status, nor previous conization for the weighted cohort design.
Overall, the strengths of this study include a collaborative effort of 126 European institutions from 29 countries where comprehensive data was collected on 1272 patients. Our project gathers one of the most extensive groups of radical hysterectomies ever collected in Europe in patients with stage IB1 cervical cancer, who underwent surgery within 2 years. We designed a strict list of inclusion and exclusion criteria to minimize the implicit bias of a retrospective study. We required mandatory preoperative imaging and a list of fundamental pathological findings for inclusion. Also, we excluded all cases with previous cone biopsy. We believe that the best way of investigating the action of a specific surgical approach in early cervical cancer is in the untouched-entire tumor even though this is still a hypothesis. Another strength of our study is the use of an inverse probability of treatment weighting based on propensity score in an attempt to balance confounders between groups, such that selection bias for the surgical procedure was minimized. We recognize that our study still has not entirely answered why minimally invasive surgery was associated with worse survival in patients undergoing radical hysterectomy. However, findings from our study on uterine manipulator and protective maneuvers are hypothesis-generating and may offer some light to the etiology of the outcomes from the LACC trial.
SUCCOR is an observational retrospective study and thus we recognize a number of limitations. However, observational studies may contribute valuable evidence supporting causal associations when designed and conducted using rigorous methods. The flaws are dependent on outdated methodology, confounding factors, heterogeneity of reporting of results, lack of replication, and a failure to interpret findings within the limitations of observational research methodology29–31 Overall, the results reflect that in this large European population findings were consistent with recently published prospective data and confirmed by several retrospective population-based analyzes published since the LACC trial.8–15
There are two prospective randomized trials exploring the role of minimally invasive surgery in patients with cervical cancer. The first is the RACC trial,28 a Swedish multicentric prospective trial comparing robotic vs open surgery for the treatment of early-stage cervical cancer. The use of uterine manipulator is not allowed, and the closure of the vagina before colpotomy is recommended but not mandatory. The second one is a multicenter randomized controlled trial designed in China.32 Again, in their protocol, the use of uterine manipulator and the method of vaginal excision is to be reported.
In summary, our study showed worse disease-free survival in patients undergoing minimally invasive radical hysterectomy when compared with the open approach. We also found that avoiding the use of the uterine manipulator or closing the vagina over the tumor might hypothetically improve the results of radical hysterectomy by minimally invasive surgery. These findings should be confirmed with prospective data. However, it is questionable whether one can genuinely repeat a prospective randomized trial in this patient population based on increasing physician hesitation to offer minimally invasive surgery or patient willingness for randomization. We conclude that in the SUCCOR study, in a European population, minimally invasive surgery was associated with significantly worse oncologic outcomes than open surgery. Avoiding the uterine manipulator and a meticulous closure of the vagina over the tumor to prevent tumor spillage may improve the outcomes of minimally invasive surgery. We look forward to the results of the ongoing randomized trials.
We thank our colleagues, Dr. Matias Jurado, Dr. Antonio Gil and Dr. Antonio Gonzalez-Martin, for their invaluable support when designing this project. A special recognition to the Medical Editor of the International Journal of Gynecological Cancer for his continuous assistance in improving this Manuscript. We thank the ESGO Council for the diffusion of the SUCCOR study among our members
Twitter @#lchiv4, @email@example.com, @RasiahBharathan, @Juan_L_Alcazar
Presented Plenary Session at the 21st European Congress on Gynaecological Oncology on November 2-5, 2019 in Athens, Greece.
Collaborators On behalf of the SUCCOR study Group: Nabil Abdalla, Sedat Akgöl, Demirkiran Aksahin, Shamistan Aliyev, Maria Alonso-Espias, Igor Aluloski, Claudia Andrade, Nikola Badzakov, Rosa Barrachina, Giorgio Bogani, Eduard-Aexandru Bonci, Hélène Bonsang-Kitzis, Felix Boria, Cosima Brucker, Laura Cárdenas, Andrea Casajuana, Pere Cavalle, Jorge Cea, Benito Chiofalo, Gloria Cordeiro, Pluvio Coronado, Maria Cuadra, Javier Díez, Teresa Diniz da Costa, Santiago Domingo, Lukas Dostalek, Fuat Elif, Diego Erasun, Mathias Fehr, Sergi Fernandez-Gonzalez, Annamaria Ferrero, Soledad Fidalgo, Gabriel Fiol, Khadra Galaal, José García, Gerhard Gebauer, Fabio Ghezzi, Juan Gilabert, Nana Gomes, Elisabete Gonçalves, Virginia Gonzalez, Frederic Grandjean, Miriam Guijarro, Frédéric Guyon, Jolien Haesen, Gines Hernandez-Cortes, Sofía Herrero, Imre Pete, Ioannis Kalogiannidis, Erbil Karaman, Andreas Kavallaris, Lukasz Klasa, Ioannis Kotsopoulos, Stefan Kovachev, Uppin Arno Leht, Arantxa Lekuona, Mathieu Luyckx, Michael Mallmann, Gemma Mancebo, Aljosa Mandic, Nabil Manzour, Tiermes Marina, Victor Martin, María Belén Martín-Salamanca, Alejandra Martinez, Gesine Meili, Gustavo Mendinhos, Liliana Mereu, Milena Mitrovic, Sara Morales, Enrique Moratalla, Bibiana Morillas, Eva Myriokefalitaki, Maja PakižImre, ImrePete, Stamatios Petousis, Laurentiu Pirtea, Natalia Povolotskaya, Sonia Prader, Alfonso Quesada, Mikuláš Redecha, Fernando Roldan, Philip Rolland, Reeli Saaron, Cosmin-Paul Sarac, Jens-Peter Scharf, Špela Smrkolj, Rita Sousa, Artem Stepanyan, Vladimír Študent, Carmen Tauste, Hans Trum, Taner Turan, Manuela Undurraga, Alicia Vázquez, Ignace Vergote, George Vorgias and Ignacio Zapardiel.
Contributors All authors contributed meaningfully to the conception or design of the work or the acquisition, analysis, or interpretation of data for the study. The authors confirm the completeness and accuracy of the data and analyses, the fidelity of the study to the protocol, and the final approval of the version to be published.
Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial, or not-for-profit sectors.
Competing interests None declared.
Patient consent for publication Not required.
Provenance and peer review Not commissioned; externally peer reviewed.
Data availability statement Data are available upon reasonable request. Data will be available upon the requirement of the reviewers at any time.