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Immunohistochemical determination of estrogen and progesterone receptors and DNA flow cytometry in endometrial cancer
  1. P. K. Heinonen*,
  2. J. Isola and
  3. T. Kuoppala
  1. * Departments of Clinical and
  2. Biomedical Sciences, University of Tampere, and
  3. Department of Obstetrics and Gynecology, University Hospital, Tampere, Finland
  1. Address for correspondence: P. K. Heinonen, MD, Department of Clinical Sciences, University of Tampere, Box 607, SF-33101 Tampere, Finland.


Estrogen and progesterone receptor contents (ER, PR) were assessed by an immunohistochemical method and DNA ploidy and S-phase by flow cytometry in frozen endometrial cancer tissue sections from 39 cases. Comparison of the immunohistochemical and cytosol assays showed 81% and 84% concordance in ER and PR contents, respectively. An aneuploid DNA pattern was identified in 30% and a high S-phase fraction was found in 33% of 36 specimens studied. Negative ER status was associated with aneuploid and high S-phase fraction. A similar association was found between PR status and high S-phase fraction. Combined analysis of immunohistochemical receptor status and DNA flow cytometry in the same sample makes it possible to identify two strong predictive factors in endometrial adenocarcinoma.

  • endometrial carcinoma
  • estrogen receptor
  • flow cytometry
  • progesterone receptor
  • S-phase fraction.

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