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1089 Expression of cytokeratin 19 in vulvar carcinomas for sentinel lymph node biopsy assessment with one-step nucleic acid amplification technique
  1. Ibon Jaunarena1,
  2. Marta Rezola2,
  3. Ainhoa Ordoñez2,
  4. Ruben Ruiz3,
  5. Manuel Moreno2,
  6. Iraide Bernal2,
  7. MIKELGorostidi Pulgar4,
  8. Miguel Angel Resano2,
  9. Juan Céspedes1,
  10. Irune Ruiz2 and
  11. Arantza Lekuona5
  1. 1Hospital Donostia, San Sebastian, Spain
  2. 2Donostia Hospital, San Sebastian, Spain
  3. 3Donostia University Hospital, Donosti, Spain
  4. 4Hospital Universitario Donostia, San Sebastian, Spain
  5. 5Donostia Osakidetza University Hospital, Donostia, Spain


Introduction/Background After the studies that demonstrate the usefulness and benefit of one-step nucleic acid amplification technique (OSNA) in sentinel lymph node biopsy (SLNB) in endometrial and cervical cancer (1–4), we wanted to evaluate the expression of cytokeratin 19 (CK19) in vulvar carcinomas in order to perform intraoperative SLNB using OSNA.

Methodology The expression of CK19 was studied in 37 paraffin tissues from vulvar biopsies diagnosed with squamous cell carcinoma from Donostia Hospital from January 2021 to December 2023.

Results Of the 37 biopsies of Donostia Hospital, 8 were CK 19 positive (22%). Among positive cases, a different rate of staining was observed ( 7–95%). None of the positive cases was associated with HPV.

A relatively different rate of CK19 expression was observed in the literature (5).

Conclusion Despite showing various positivities and in some cases of low rate, the fact of its existence makes considering the study of CK19 with immunohistochemistry in a protocol manner in biopsies of vulvar squamous cell carcinoma; Although special caution and prudence should be taken in cases with a low percentage of expresión, patients who show diffuse positivity could benefit from the intraoperative SLNB study with OSNA, increasing the detection of micrometastases, and avoiding the complications derived from a second intervention.

Disclosures No.

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