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234 Immunohistochemical analysis of P53 in vulvar intraepithelial neoplasia and vulvar carcinoma
  1. Sabina Murshudova
  1. National Center of Oncology, Baku, Azerbaijan

Abstract

Introduction/Background Vulvar cancer accounts for significant patient morbidity and mortality, particularly in the setting of recurrent or treatment refractory disease. p53 inactivation due to oncogenic viral proteins or mutations is an important molecular mechanism in vulvar carcinogenesis. To evaluate p53 changes in vulvar carcinogenesis, we analyzed p53 expression in vulvar carcinoma (VC), vulvar intraepithelial neoplasia (VIN), lichen sclerosus (LS) and squamous cell hyperplasia (SH).

Methodology 23 cases of VC, 36 cases of VIN, 32 samples of LS with 4 associated to carcinoma, 38 biopsies of SH with 6 associated to carcinoma and 10 cases without neoplastic changes were stained immunohistologically for p53. The pattern, intensity and number of stained cells were analyzed. Results were compared to p53 expression in vulvar epithelium without neoplastic changes.

Results Normal vulvar epithelium showed low p53 staining in the basal epithelial layers. 35% of LS and SH not associated to carcinoma showed immunohistological signs of p53 mutation, while cases associated with carcinoma did so in 85%. In VIN, p53 was predominantly overexpressed in the differentiated subtype. 72% of VC showed immunohistological changes of p53 expression.

Conclusion p53 mutations are present in typical carcinomas, precursor lesions and disorders with elevated risk for vulvar cancer. Thus, p53 mutation seems to occur early in vulvar carcinogenesis and may become a useful marker, especially in lesions with increased risk of cancer.

Disclosures No.

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