Introduction/Background With the cervical cancer national screening program in Turkey, patients aged 30–65 are screened on a population basis with HPV-DNA testing. According to the latest guidelines, patients with non 16–18 high risk HPV (hrHPV) and negative cervical cytology tests are followed up annually. We aimed in this study to contribute to the management of non 16–18 hrHPV positive patients by evaluating the relationship between non 16–18 hrHPV and premalignant lesions.

Methodology The colposcopic results of women aged 30–65 who were determined HPV-DNA positive and were referred to our hospital from Cancer Screening Centers between 2019 and 2023 were retrospectively scanned. HPV DNA samples were tested using Hybrid capture 2 (Qiagen, Germany), a technology that performs nucleic acid hybridization with signal amplification using the microplate chemiluminescence method. Colposcopy examinations were performed by colposcopists at the gynecological oncology clinic, and biopsies were taken when deemed necessary. We determined the rates of high-risk lesions in patients with non 16–18 hrHPV positive test with negative and positive cytology.

Results 602 patients with non 16–18 hrHPV positive tests were included in the study. Among 472 patients with non 16–18 hrHPV positive and normal cervical cytology tests, 55 had high-grade lesions. 53 of these patients had CIN 3, 1 had microinvasive squamous carcinoma and 1 had adenocarcinoma . In this study, colposcopic biopsy results of 89.6% of the patients with non 16–18 hrHPV positive test and negative cytology were normal. However, 10.4% of these patients with negative cytology were still CIN3 and CIN3+.

Conclusion Although the association of non 16–18 hrHPV positive patients with cervical cancer is not as high as HPV 16–18, it should still be considered that up to 10% of patients with normal cervical cytology may have an underlying high-grade premalignant lesion.

Disclosures If we think that patients follow-up will be difficult, performing colposcopy immediately appears to be acceptable.