Article Text

Download PDFPDF
768 Non-inferiority study of the OSNA technique with respect to ultrastaging in the sentinel lymph node study in cancer of the initial cervical
  1. Maria Teresa Climent Marti1,
  2. Anna Serra Rubert1,
  3. Mar Bort Gimeno1,
  4. Teresa Arrufat Conesa1,
  5. Maria Gervilla Diaz2 and
  6. Antoni Llueca Abella1
  1. 1University General Hospital of Castellon, Castellon, Spain
  2. 2University of Jaume I, Castellon, Spain


Introduction/Background Lymph node involvement is one of the main prognostic factors in cervical cancer, where sentinel node study currently associated with systematic pelvic lymphadenectomy is essential.

Several studies point to the one-step nucleic acid amplification (OSNA) technique, already applied in breast cancer, as an effective, fast, and economical alternative for the study of sentinel lymph node in cervical cancer in early stages.

To perform a systematic review and meta-analysis demonstrating the non-inferiority of the OSNA technique with respect to ultrastaging in the study of sentinel lymph node in early cervical cancer.

Methodology After a literature search, 4 prospective and retrospective studies analyzing OSNA in early cervical cancer are included. OSNA is compared with conventional histology in two of them, as well as with Ultrastaging in the remaining two. Sensibility (S), specificity (E) positive predictive value (PPV) and negative predictive values (NPV), Kappa Index and diagnostic accuracy were extracted and integrated in

Forest Plot and ROC curves and statistically compared.

Results The OSNA technique is a tool comparable to conventional histology, with a diagnostic accuracy of 98%. In addition, it establishes a Kappa index with respect to ultrastaging of 0.6 (95% CI=0.45–0.75), and higher detection rate of micrometastases.

Conclusion OSNA is proposed as an effective technique for sentinel node analysis in cervical cancer, with application in intraoperative setting and higher detection rate of small volume metastases with respect to histology and ultrastaging, being necessary more studies to confirm the described trend.

Disclosures I have no financial disclosure or conflicts of interest with the presented material.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.