Introduction/Background Lymph node involvement is one of the main prognostic factors in cervical cancer, where sentinel node study currently associated with systematic pelvic lymphadenectomy is essential.

Several studies point to the one-step nucleic acid amplification (OSNA) technique, already applied in breast cancer, as an effective, fast, and economical alternative for the study of sentinel lymph node in cervical cancer in early stages.

To perform a systematic review and meta-analysis demonstrating the non-inferiority of the OSNA technique with respect to ultrastaging in the study of sentinel lymph node in early cervical cancer.

Methodology After a literature search, 4 prospective and retrospective studies analyzing OSNA in early cervical cancer are included. OSNA is compared with conventional histology in two of them, as well as with Ultrastaging in the remaining two. Sensibility (S), specificity (E) positive predictive value (PPV) and negative predictive values (NPV), Kappa Index and diagnostic accuracy were extracted and integrated in

Forest Plot and ROC curves and statistically compared.

Results The OSNA technique is a tool comparable to conventional histology, with a diagnostic accuracy of 98%. In addition, it establishes a Kappa index with respect to ultrastaging of 0.6 (95% CI=0.45–0.75), and higher detection rate of micrometastases.

Conclusion OSNA is proposed as an effective technique for sentinel node analysis in cervical cancer, with application in intraoperative setting and higher detection rate of small volume metastases with respect to histology and ultrastaging, being necessary more studies to confirm the described trend.

Disclosures I have no financial disclosure or conflicts of interest with the presented material.