Introduction/Background Despite diligent efforts in primary and secondary prevention, cervical cancer (CC) persists as a prevalent malignancy among women, necessitating the exploration of novel prognostic and predictive factors for practical application. Existing literature suggests a correlation between the expression levels of Reactive Oxygen Species Modulator 1 (ROMO 1) and the disease stage. The objective of the retrospective study was to establish the potential association between the ROMO 1 expression levels and the histological type and the lymph node status in CC patients.

Methodology For each participant, a 3 µm-thick section from the corresponding formalin-fixed and paraffin-embedded tumor block underwent staining for ROMO1 (Clone OTI2C12, Mo, dilution 1:150, Abcam, UK). The immunohistochemical procedure employed the EnVision™ FLEX, High pH (DAKO) system and the AutostainerLink 48 technique (DAKO). The H score was utilized due to the absence of an established scoring system for ROMO 1.

Results This retrospective study, encompassing the years 2015–2020, encompasses a cohort of 150 CC patients who received treatment at the Department of Oncogynecology of Medical University Pleven (see table 1).

From the studies, no statistically significant difference was found in the expression of ROMO 1 in patients N0 and N1 (p=0.969), but when we analyzed the relationship with the histological types statistically significant difference was noted (p=0.002).

Conclusion ROMO1 expression was highest in SCC, followed by ASC and AC and there is no significant difference in expression levels according to lymph node status in CC patients.

Disclosures The authors have no relevant financial or non-financial interests to disclose.