Article Text

Download PDFPDF

EP032/#1390  Tumor-infiltrating lymphocytes: a prognostic biomarker in neoadjuvant-treated HR positive/HER2 negative breast cancer
  1. Yilong Liu,
  2. Changyu Zhu and
  3. Rongsheng Tong
  1. University of Electronic Science and Technology of China, School of Medicine, Chengdu, China


Introduction Anticancer immune responses contribute to the success of chemotherapy. We aimed to elucidate the value of tumor-infiltrating lymphocytes (TILs) as a prognostic marker for distant relapse-free survival (DRFS) in patients with HR positive/HER2 negative breast cancer in the taxane/anthracycline-based neoadjuvant chemotherapy (NACT).

Methods The cell type enrichment analysis for 64 immune and stromal cell types was performed with the web-based tool xCell based on RNA expression profiles of breast cancer from National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO). Kaplan-Meier analysis and LASSO-Cox PH regression model were used to assess the correlation of TILs and stromal cells infiltration with breast cancer distant relapse-free survival.

Results In this study, 123 HR positive/HER2 negative breast cancer patients derived from the dataset GSE25055 were eventually enrolled in the present study. LASSO-Cox PH regression analysis demonstrated that pre-NACT plasma cells and Th2 cells infiltration exhibited an independent prognostic value for DRFS (HR = 11.26, P = 0.036; HR = 15.13, P <0.001; respectively). A risk scoring model based on the TILs was conducted to divide patients into different risk groups with significantly different DRFS rates (P=0.0028). Compared with low risk group, high-risk group was comparatively associated with worse DRFS rates (3-year DRFS rate, 72.3% vs. 93.2%, P=0.0028).

Conclusion/Implications These results suggested that pre-NACT immunological plasma cells and Th2 cells infiltration is an independent predictive factor of DRFS for the patients with HR positive/HER2 negative breast cancer, which provides valuable and profound perspective of immune microenvironment and NACT prognosis.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.