Introduction WHO recommends HPV self-sampling as a safe and highly accepted additional strategy in cervical cancer screening for women aged 30–60 years [1]. Currently, no research on HPV self-sampling has been conducted in Vietnam. This study aims to evaluate the accuracy, acceptability, and women’s experience of HPV self-sampling compared to healthcare provider collection at Da Nang Oncology Hospital.

Methods A cross-sectional study was conducted at Da Nang Oncology Hospital from April to June 2023 for women aged 30–65 years. HPV self-sampling was performed using Copan Self Vaginal FLOQSwabs prior to physician-collected HPV on the same day. Samples were preserved in Thinprep PreservCyt and analysed by Roche Cobas 4800.

Results The study included 108 cases, for which the sample-inadequacy rate was 4.6% (5/108 cases). Patient’s mean age was 44.0 ± 8.1 with 75.0% aged 30–49 years. Among 103 qualified cases, positive rates for HPV16, HPV18 and 12 other high-risk HPV types were 0.97%, 0% and 2.91% respectively in self-collected versus 0.97%, 0.97% and 2.91% in physician-collected samples. The accuracy of HPV self-sampling was 99.0% when using physician-sampling as a reference (102/103 cases). 50.0% of women preferred self-collection, 44.4% preferred physician-collection, and 5.6% had no preference. 77.8% believed healthcare provider-sampling was more accurate than self-sampling. Only 8.3% reported painful experience and 9.3% encounted difficulty with self-sampling. The majority (91.7%) would choose HPV self-sampling at home for next screening and 96.3% would recommend it to other women.

Conclusion/Implications HPV self-sampling is an accurate and highly acceptable approach for Vietnamese women to improve the cervical cancer screening rate.