Article Text

Download PDFPDF

EP199/#26  ATR-FTIR spectroscopy analysis of gynecological tumor paraffin blocks
  1. Ariel Polonsky,
  2. Hila Weinberger,
  3. Moran Gawie- Rotman,
  4. Fatme Kedan and
  5. Ilan Bruchim
  1. Hillel Yaffe medical center, Ob/gyn, Hadera, Israel


Introduction Epithelial ovarian cancer (EOC) is the most lethal cancer among gynecological malignancies worldwide, accounting for 90% of all ovarian cancers. Our study’s primary objective is to discriminate benign from malignant ovarian tumors using tissue samples that underwent formalin fixation using ATR-FTIR spectroscopy.

Methods This is a retrospective, single center study. Inclusion criteria were of formalin fixed tissue taken from pathology archive samples, resected from females ages 18 and above. Two sets of slides from malignant tumors and 2 sets of slides from benign tumors were used to study the effect of tissue thicknesses on the measured absorption spectra: a set of 4 microns and a set of 12 microns from each tumor type were compared. Spectroscopic measurements were performed on the different slides. The 4 microns tissue thickness group was chosen. Each slide was measured in multiple locations. PCA-LDA Discrimination analysis was performed using the measured spectra. The cross-validation process was repeated five times. The results of these validations were then averaged to produce a single estimation.

Results A total of 74 tissue samples were examined. Absorption spectra of the malignant tumors were consistently different from that of benign tumors at many spectral ranges. Using K-fold cross validation technique, the study showed that the model correctly classified the samples into malignant and benign groups with an accuracy of 94.5%.

Conclusion/Implications Our study exhibits a sensitive method to differentiate between a benign and malignant paraffin block preparation. With further research, this technique could become an alternative to conventional histopathology.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.