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Abstract
Introduction As a critical position of the multilevel gene expression regulation program, translational regulation has been found to be widely involved in cancer initiation and progression. Cervical cancer is the second largest female malignant tumor in China. The high-resolution and genome-wide view of the landscape of RNA translation in cervical cancer is still limited.
Methods We performed the ribosome profiling for 25 samples of human cervical cancer at various stages and 10 samples of nomal cervical tissues, respectively. A series of bioinformatics tools was then utilized to these data for the mining of novel insights into the translational dysregulation in cervical cancer.
Results This is the first translatome data resource for dissecting dysregulated translation in cervical cancer at the sub-codon resolution. Our data suggested that there are significant differences in the transcriptome and translationome in cervical cancer initiation and progression. For example, multiple proteins involved in cell cycle or cell-cell adhesion exhibited significant translational upregulation and downregulation in cervical cancer when comparing with normal tissue (figure 1). In addition, we analyzed the translatome data with clinical features, which shown translational dysregulation leading to immune dysregulation may be an important reason for the progression of cervical cancer (figure 2).
Conclusion/Implications This study has constructed the first translatome of cervical cancer, which provides a valuable data resource and novel insights in cervical cancer initiation and progression.
Translation landscape
Analyzed the translatome data with clinical features