Introduction/Background Cervical HPV infection is a proven cause of precancer and cervical cancer. The aim of our study is to establish the presence and genotypes of HPV in Bulgarian patients with invasive cervical cancer.

Methodology The study was carried out retrospectively using the results database of the Department of General and Oncogynecology at the Military Medical Academy (MMA), Sofia, Bulgaria. The results from a four-year period (2018–2022) were studied. We included 31 women in the study, aged 18- 45 years, who attended a gynecological clinic for surgical treatment of histology proven (by biopsy or abrasion) invasive cervical cancer (FIGO Ib – IIb stages). HPV cervical screening (to determine the presence or absence of infection) during a routine gynecological preoperative check-up was done. HPV typing (GenoFlow HPV Array Test Kit) was performed to detect 33 highs, moderate and low - risk HPV genotypes. Cervical swabs were taken and analyzed at the Department of Virology – MMA. Interpretation of the results was done using data analysis strips and an image capture system for scanning, analysis and storage of the membrane image. Statistical analysis was performed using IBM SPSS Statistics. All patients included in the study gave their informed consent and the study was approved by the MMA ethical committee.

Results The obtained results showed that, one/more HPV genotypes were identified in 32.3% (10/31) and HPV (-) in 67.7% (21/31) of the examined women. The statistical analysis did not reveal sufficient positive predictive value or sensitivity (p > 0.05), i.e. probability that selected cervical cancer patients are HPV (+).

Conclusion These results indicate that cervical infection with HPV is probably not the only cause for development of invasive cervical cancer or it cannot be detected for other reasons.

Disclosures Authors have nothing to disclosures