Introduction/Background The aim of this study is to evaluate the presence of specific microbiota in cervical cancer patient in material collected from endocervical canal (cervical microbiota). The results will be a basis for further research on cervical cancer co-factors which may stimulate carcinogenesis.

Methodology 20 women diagnosed with cervical cancer independently of FIGO stage, were included in the study. Before the treatment (surgery, CRTH) sample from the endocervical canal was collected using cytology brush and preserved in Liquid-Based Cytology container. All collected samples were analyzed to isolate DNA with a NucleoSpin® Tissue kit. DNA isolates were subjected to qualitative and quantitative evaluation, using libraries based on the Illumina-16S Metagenomic Sequencing Library Preparation (16S Sequencing) protocol.

Results The mena age of participant was 54.23 years (range: 32-89 years, median 51 years). Nine women were postmenopausal, eleven were in reproductive age. In both groups bacterial vaginosis associated bacteria (Prevoletta spp., Gardnerella vaginalis group, Atopobium vaginae) were dominant part of vaginal microbiota. In two patients in reproductive age with rapid progression and advanced stage Fusobacterium nucleatum was dominating. Interestingly, the prevalence of Ureaplasma spp and Mycolpasla spp in this group was low.

Conclusion In the population of premenopausal women diagnose with cervical cancer presence of Fusobacterium nucleatum in cervical microbiota might be an indicator of rapid progression and poor prognosis. Further studies are need to confirm that observation.

Disclosures None