Introduction/Background Previously we reported that lipolysis-stimulated lipoprotein receptor (LSR) mediates the cell proliferation via lipid metabolism in epithelial ovarian cancer. Our newly anti-LSR antibody demonstrated stronger anti-tumor effect against LSR positive epithelial ovarian cancer cells in High-Fat Diet (HFD) fed mouse compared to Normal-Diet (ND) fed mouse. That suggests lipid molecules in HFD might contribute to cell proliferation via LSR. In this research, we performed metabolome analysis using HFD and ND fed mouse serum. We analyzed the metabolomic profile of lipid molecules.

Methodology We established HFD and ND fed mouse and obtained each serum to perform metabolome analysis. We compared the metabolomic profile of HFD and ND fed mouse serum and identified the lipid metabolites which might associate with the cell proliferation. We evaluated the effect of lipid molecules on promoting cell proliferation and signaling pathway mediated via LSR.

Results Metabolome analysis detected 210 metabolites, and PCA showed obviously different metabolic profiles of HFD mouse serum compared to ND mouse serum. PLS-DA also revealed cholesterol, oleic acid and arachidonic acid as lipid molecules with high VIP score. We revealed that these molecules significantly promoted the cell proliferation by cell proliferation assay (p<0.05) and these molecules activated the MAPK signaling pathway.

Conclusion Metabolome analysis identified the lipid molecules which are associated to the cell proliferation of LSR positive epithelial ovarian cancer.

Disclosures I have no potential conflict of interest to report.