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Poster/ePoster Sessions
06. Ovarian cancer
#796 mirna panel failed to distinguish between EAOC (endometriosis associated ovarian cancer) and high grade ovarian cancer
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  1. Maria Szubert1,
  2. Anna Nowak-Glueck1,
  3. Daria Domanska-Senderowska2,
  4. Bozena Szymanska3,
  5. Aleksander Rycerz4,
  6. Piotr Sowa5 and
  7. Jacek Wilczynski6
  1. 1Department of Surgical and Oncologic Gynecology, 1st Department of Gynecology and Obstetrics, Medical University of Lodz, Poland; M. Pirogow’s Teaching Hospital, Wilenska 37 St.,, Lodz, Poland
  2. 2Department of Molecular Medicine, Medical University of Lodz, Pomorska 251 ST., 92–213 Lodz, Poland, Lodz, Poland
  3. 3Central Scientific Laboratory CoreLab, Medical University of Lodz, Mazowiecka 6/8 St., 92–215 Lodz, Lodz, Poland
  4. 4Department of Surgical and Oncologic Gynecology, 1st Department of Gynecology and Obstetrics, Medical University of Lodz,, Lodz, Poland
  5. 5Department of Pathology, M. Pirogow’s Teaching Hospital, Wilenska 37 St.,, Lodz, Poland
  6. 6Department of Surgical and Oncologic Gynecology, 1 st Department of Gynecology and Obstetrics, Medical University of Lodz,, Lodz, Poland

Abstract

Introduction/Background Endometriosis is one of the most common gynaecological diseases affecting approximately 6–10% of women in reproductive age. Despite its benign character it poses a 0,7–1% risk of malignant transformation particularly due to ovarian endometriosis. Endometriosis associated ovarian cancer (EAOC) consisting of endometrioid cancer and clear cell ovarian cancer could be promoted by many factors. miRNAs which are small, non-coding molecules of RNA are among them. The aim of this study was to detect miRNAs connected with malignant transformation of endometriosis and to assess if miRNA panel can be helpful in distinguishing ovarian cancers according to their pathological origin.

Methodology FFPE (formalin fixed paraffin embedded) of 179 patients operated on endometriosis and different types of ovarian cancer were studied. High grade ovarian cancer was choosen as comparator. From expression panel of 754 miRNAs, 7 were identified for further tests according to their ROC curves and at least 2-fold change in expression comparing to the reference gene: miR-1–3p, miR-125b-1–3p, miR-31–3p, miR-200b-3p, miR-502, miR-503, miR-548d. Furthermore, other potentially important clinical data were analysed, which included: age, BMI, Ca125 concentration, FIGO stage, miscarriages and deliveries, concomitant diseases such as hypertension, type 2 diabetes and smoking.

Results Examined miRNA panel distinguished significantly different types of endometriosis, normal ovarian tissue and endometriosis, normal ovarian tissue and cancer, endometriosis and cancer. miRNA expression was not dependent on FIGO stage or Ca125 concentration. The panel has no potential to differentiate types of ovarian cancer according to their origin.

Conclusion Transformation in malignant cells are dependent on expression of different miRNA of pleiotropic function, therefore examination of broader miRNA panel and correlation between different miRNA molecules is needed and might prove itself advantageous in clinical practise.

Disclosures as attached

https://doi.org/10.1136/ijgc-2023-ESGO.646

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