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#646 Gene expression of membrane transporters: importance for prognosis and progression of ovarian carcinoma
  1. Martin Hruda1,2,
  2. Katerina Elsnerová3,2,4,
  3. Beatrice Mohelníková Duchonová3,5,
  4. Ela Cerovská3,6,
  5. Marie Ehrlichová3,4,
  6. Ivan Gut3,
  7. Lukáš Rob1,2,
  8. Petr škapa7,
  9. Alena Bartáková8,
  10. Jirí Bouda8,
  11. Pavel Vodicka4,9,
  12. Pavel Soucek3,4 and
  13. Radka Václavíková3
  1. 1University Hospital Královské Vinohrady, Prague, Czech Republic
  2. 2Third Faculty of Medicine, Charles University, Prague, Czech Republic
  3. 3Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic
  4. 4Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic
  5. 5Palacký University and University Hospital Olomouc, Olomouc, Czech Republic
  6. 6Faculty of Science, Charles University, Prague, Czech Republic
  7. 7University Hospital Motol, Prague, Czech Republic
  8. 8Faculty of Medicine and University Hospital in Pilsen, Pilsen, Czech Republic
  9. 9Department of the Molecular Biology of Cancer, Institute of Experimental Medicine, Academy of Science, Prague, Czech Republic


Introduction/Background Membrane transporter proteins (for example ABC, SLC and ATPases) take part in oncogenesis and the formation of chemoresistance, however the interpretation of their importance in ovarian cancer prognosis is still limited.

Methodology Gene expression profiling was performed on 39 ABC and 12 SLC transporters and 3 ATPases in epithelial ovarian cancer (EOC) tissues and the results were assessed with respect to prognosis and clinical presentation of EOC patients. In a cohort of tissues with primary EOC (n = 57) and control tissues (n = 14) we assessed relative expression of genes and compared it with the clinical and survival information. The data was verified on a separate cohort (n = 60).

Results 6 ABC genes and the SLC22A18 gene were considerably over–expressed in the cancer tissues in comparison with control tissues and the expression of 12 ABC genes, 5 SLC genes, ATP7A, ATP11B was reduced. The expression of ABCA12, ABCC3, ABCC6, ABCD3, ABCG1, SLC22A5 genes was greater in HGSC in comparison with other types. Expression of ABCA2 was considerably related to tumour grade in both cohorts. Particularly, the expression levels of ABCA9, ABCA10, ABCC9 and SLC16A14 were considerably related to PFS in both pilot and verification groups. ABCG2 level was related to PFS in the grouped patient cohort.

Conclusion The genes ABCA2, ABCA9, ABCA10, ABCC9, ABCG2 and SLC16A14 represent new potential markers of epithelial ovarian cancer progression and collectively with the discovered association between the expression of ABCA12, ABCC3, ABCC6, ABCD3, ABCG1, SLC22A5 and HGSC they should be investigated more extensively in future studies.

Disclosures This study was supported by running projects of the Czech Health Research Council grant no. NU22–08-00186 and Cooperatio program no. 207035, ‘Maternal and Childhood Care’ by 3rd Faculty Medicine, Charles University.

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