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MicroRNA expression as a diagnostic parameter in early endometrial cancer
  1. Stefan Blagojević1,
  2. Branko Andrić2,
  3. Jovana Jovankić1,
  4. Milena Milutinović1,
  5. Danijela Nikodijević1,
  6. Petar Arsenijević3 and
  7. Danijela Cvetković3
  1. 1 University of Kragujevac, Faculty of Science, Kragujevac, Serbia
  2. 2 Health Center Raška, Raška, Serbia
  3. 3 University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia
  1. Correspondence to Stefan Blagojević, Department of Biology and Ecology, University of Kragujevac, Faculty of Science, Kragujevac, Serbia; stefan.blagojevic{at}pmf.kg.ac.rs

Abstract

Objectives MicroRNAs (miRNAs) have emerged as biomarkers that showed strong diagnostic potential in various diseases, including cancer. This study aimed to estimate the expression and diagnostic potential of miRNAs (miR-200a, miR-21, miR-210, miR-126, and miR-130a) in endometrial cancer samples. The DICER1 and AGO2 genes were also analysed.

Methods The expression of miRNAs, DICER1, and AGO2 was quantified using the quantitative real-time PCR method in 40 tissue samples with early-stage endometrial cancer and 16 normal controls.

Results All tested miRNAs showed significantly higher expression in endometrial cancer compared with the control group, while DICER1 was significantly downregulated. The expression levels of miR-200a, miR-21, and miR-210 were negatively correlated with DICER1 expression. Individually, miR-200a, miR-21, miR-210, and DICER1 showed the best diagnostic performance in distinguishing patients with endometrial cancer from normal controls, whereas a combination of all biomarkers resulted in an even higher area under the curve.

Conclusions Our study showed that a panel of selected biomarkers (miR-200a, miR-21, miR-210, miR-126, miR-130a, DICER1, and AGO2) may be candidates for the detection of early-stage endometrial cancer.

  • Uterine Cancer

Data availability statement

The datasets used in this study are available from the corresponding author on reasonable request.

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Data availability statement

The datasets used in this study are available from the corresponding author on reasonable request.

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Footnotes

  • Contributors Stefan Blagojević: Conceptualization, Methodology, Validation, Formal analysis, Investigation, Writing - Original Draft, Visualization, Writing - Review and Editing, Project administration. Branko Andrić: Conceptualization, Investigation, Resources, Data Curation, Writing - Review and Editing. Jovana Jovankić: Conceptualization, Methodology, Investigation, Writing - Review and Editing, Visualization. Milena Milutinović: Conceptualization, Resources, Writing - Review and Editing. Danijela Nikodijević: Conceptualization, Writing - Review and Editing. Petar Arsenijević: Conceptualization, Resources, Supervision, Project administration. Danijela Cvetković: Guarantor, Conceptualization, Methodology, Validation, Resources, Writing - Review and Editing, Visualization, Supervision, Project administration. All authors read and approved the final version of this manuscript.

  • Funding The work was supported by the Ministry of Science, Technological Development and Innovation of the Republic of Serbia (Agreement no. 451-03-47/2023-01/200122 and no. 451-03-47/2023-01/200111). The authors declare that no other funds, grants, support were obtained for this study.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.