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Abstract
Objective In vitro maturation of oocytes collected from oophorectomy samples might be a promising approach in the field of oncofertility. In this study, we evaluate the feasibility of in vitro maturation of oocytes collected from oophorectomy samples in patients with ovarian tumors.
Methods This prospective observational study included 27 patients with malignant ovarian tumors. Patients underwent oophorectomy and ovarian tissue was examined for the presence of immature cumulus-oocyte complexes. These were matured in vitro for 48 hours. Mature oocytes were vitrified or used for fertilization. Serum anti-müllerian hormone (AMH) levels were analyzed in 11 patients and cancer antigen 125 (CA125) levels in 16 patients.
Results In this study, 99 cumulus-oocyte complexes were obtained from 17 patients (63%). The mean (SE) age of the patients was 33.47±1.86 years (range 16–44). A total of 14 patients had ovarian cancer (IA–IVB), one patient had ovarian cancer IC and endometrial cancer IA, one patient had endometrial cancer stage IA with metastasis into the ovary, and one patient had cervical cancer stage IIB with metastasis in the ovary. Oocytes were not obtained in 10 patients who had diminished ovarian reserve due to age (>38 years), chemotherapy, or previous surgical treatment. On average, 5.8 cumulus-oocyte complexes were obtained per patient. The maturation rate was 40.4% with an average of 2.8 metaphase II oocytes per patient. As a result of the study, 3 blastocysts in 3 patients and 22 oocytes in 9 patients were vitrified.
Conclusions In vitro maturation of oocytes collected from oophorectomy samples in patients with malignant ovarian tumors may result in oocyte and blastocyst vitrification. However, it should be offered to patients before surgery and chemotherapy. This method might be most beneficial in patients younger than 38 years, with AMH serum levels >1 ng/mL and without a large tumor burden.
- ovarian cancer
- ovariectomy
Data availability statement
Data are available upon reasonable request. In accordance with the journal’s guidelines, we will provide our data for the reproducibility of this study in other centers if such is requested.
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Data availability statement
Data are available upon reasonable request. In accordance with the journal’s guidelines, we will provide our data for the reproducibility of this study in other centers if such is requested.
Footnotes
EB and AK contributed equally.
Correction notice This article has been corrected since it first published. The provenance and peer review statement has been included.
Contributors AK, AA, and GT developed the OTO IVM procedure. AA supervised the OTO IVM procedures, EB, NM and TN were responsible for the clinical management of the patients, EB collected the patients’ medical history, AK performed the laboratory procedures, GK was responsible for the patients’ diagnostic evaluation and performed the surgeries, AlA performed the histological evaluation and cancer staging, EB, AK, and AlA wrote the paper. All authors reviewed the manuscript. EB and AK contributed equally.
Funding AK and EB were supported by the Grant of the President of the Russian Federation - for state support of young Russian scientists - К-3244.2019.7.
Competing interests None declared.
Provenance and peer review Not commissioned; externally peer reviewed.
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