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426 The role of KI-67, P16 and BCL-2 in the management of cervical neoplasia
  1. Anca Copos,
  2. Diana Mocuta,
  3. Cristina Aur and
  4. Romina Cuc
  1. County Emergency Clinical Hospital of Oradea; University of Oradea, Faculty of Medicine; Obstetrics – Gynecology


Introduction/Background Cervical cancer, a common gynecological tumor, has a high mortality and it seriously threatens the health of the women.The biomarkers of cell proliferation and apoptosis indicate the early carcinogenesis and are useful for future patient monitoring. HPV persistent infection causes overexpression of P16, but this could be also in normal tissue. P16 is important and useful for cervical cancer screening, but combined with other biomarker - Ki-67, which is a marker of cell proliferation. In normal tissues, the simultaneous expression of P16 and Ki-67 is less likely to occur. Bcl-2 is an intracellular membrane protein which prevents apoptotic cell death and it can be used as a biomarker, too.

Methodology We selected a number of 40 paraffin embedded specimens of cervical tissue from patients diagnosed with cervical pathology, who were admitted in our department from 1-st of January 2018 till 31-th of December 2019. The specimens groups were formed by L-SIL (10), H-SIL (10), scuamous cervical carcinoma – SCC (10) and nontumoral cervical tissue (10) as control group. For all the specimens was performed the histopatological exam and the immunohistochemistry for Ki-67, P16 protein and Bcl-2 protein. Expression of Ki 67, P16 protein and Bcl-2 was detected and the diagnostic values were analyzed.

Results Positive rates of Ki 67 and P16 expression in H-SIL and SCC groups were significantly higher than those in L-SIL and control group. In our study the expression’s intensity of P16 and Ki-67 was positively correlated with the degree of cervical lesions.The immunostaining for Bcl-2 is highly expressed in cervical cancer tissue, compared with nontumoral cervical tissue. The difference is not well expressed compared to H-SIL and L-SIL.

Conclusion Cervical cancer is the malignant tumor with a known etiology, so that prophylactic measures could be taken.The combination of P16 and Ki-67 can identify patients with high risk of SCC and reduce the rate of misdiagnosis. This is of high value for the differential diagnosis between SCC and H-SIL. Bcl-2 is an important regulator of apoptosis. The relationships of tumor genesis with anti-apoptotic genes and pro-apoptotic genes have been confirmed. Combined with other biomarkers, Bcl-2 could be usefull in assesing the patients’ prognosis.

Disclosures I have nothing to disclose.

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