Methodology This study was approved by the Institute Research Medical Ethics Committee. The study involved 62 patients vaccinated against HPV, aged 19 to 45 years. The patients were divided into 2 groups: group 1 - HPV-positive (n = 14) and group 2 - HPV-negative (n = 48). CVF proteomic analysis was carried out by the bottom-up method (HPLC-MS/MS) on an Agilent 1100/7 Tl LTQ-FT Ultra mass spectrometer (Thermo Electron, Germany). The search for protein identification and semiquantitative analysis was carried out against the UniProt Knowledgebase (UniProtKB, version 16.05.2014) using the MaxQuant software, version 18.104.22.168.
Results None of the vaccinated women showed cervical pathology by cytology. In 6 cases, a biopsy of the cervix was performed in the presence of HPV carcinogenic risk together with mild changes in the cervix epithelium during colposcopy. The histological diagnosis in all cases (n = 6) corresponded to CIN1.
In total, more than 419 different proteins were detected in CVF samples. The level of 34 CVF proteins were significantly different in HPV positive group compared to HPV negative patients (p<0.05). Among them, 9 proteins were involved in the innate immunity processes (APOB, FABP5, GRN, HP, MUC5AC, OLFM4, PKP1, QSOX1, S100A8).
Conclusion A high incidence of HPV was revealed in Russian women previously vaccinated against HPV (23%). CIN 1 was detected in 6 HPV-positive vaccinated women (43%) with normal cytology (NILM). Proteomic analysis of CVF can be proposed as a non-invasive method for detecting biomarkers for early diagnosis of CIN. This work was supported by RSF grant No. 18-75-10097.
Disclosures Nothing to disclose.
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