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EP1026 CD4+IL-21+ and IL-22+ and CD4+FOXP3 in a algorithm ROMA relationship in patients affected by ovarian tumours
  1. I Winkler1,2,
  2. J Woś3,
  3. A Karczmarczyk4,
  4. P Miotła2,
  5. M Gogacz2,
  6. K Skorupska2,
  7. T Rechberger2 and
  8. J Tabarkiewicz5
  1. 1II Department of Gynecology, St’Johns Center Oncology
  2. 2II Department of Gynecology
  3. 3Department of Clinical Immunology
  4. 4Department of Experimental Hematooncology, Medical University in Lublin, Lublin
  5. 5Centre for Innovative Research in Medical and Natural Sciences, Medical University in Rzeszów, Rzeszów, Poland


Introduction/Background The aim of the study was to estimate the relationship of Treg (CD4+ FOXP3+) and Th17 cells producing IL21 and IL-22 in the peripheral blood and the tissue of the epithelial ovarian tumor, to blood serum levels of markers HE4 and CA125 and to assess the application of the risk of ovarian malignancy algorithm (ROMA).

Methodology Mononuclear cells (PBMCs) obtained from the peripheral blood and the ovarian tissue of patients suffering from ovarian pathology were isolated by density gradient centrifugation. The control group consisted of patients who had undergone surgery for unexplained infertility. The percentage of Treg and Th17 producing IL-21+ or IL-22+ lymphocytes in peripheral blood and tissue was assessed using the flow cytometry method according to the manufacturer’s instructions (figure 1).The ROMA index was calculated by way of levels of HE4 and CA125 in serum.

Abstract EP1026 Figure 1

Representative dot plots illustrating the analysis method for the identification of Treg c

Results A larger concentration of marker HE4 and value of ROMA was shown in patients with ovarian cancer, when compared with women without ovarian pathology and cystadenomas. A negative correlation was also found in the percentage of CD4+/IL-21+ in the peripheral blood and the amount of Treg infiltrating normal ovarian tissue. Moreover, we observed a relationship between the ROMA percentage in the serum and Treg in the peripheral blood of women suffering from benign ovarian tumors (figure 2).

Abstract EP1026 Figure 2

The relationship between serum ROMA percentage and Treg in the peripheral blood of women s

Conclusion In patients with benign tumors, we found for the first time, significant negative correlation between percentages of circulating Treg cells in the peripheral blood and with ROMA assessment in the serum. This result could be explained by the negative influence of Treg on inflammation and secondary on malignancy induced by chronic inflammation. Furthermore, the imbalance in Treg percentage in normal ovarian tissue of patients suffering from unexplained infertility, could induced immunotolerance, and hence, infertility.

Disclosure Nothing to disclose.

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