Introduction/Background The alpha parameter characterizes the ratio of single-stranded and double-stranded nucleic acids in the lymphocyte. The values of this parameter can be used as a signal test containing generalized information about the complex of changes in the body of a cancer patient. The purpose of the study was to perform a luminescent-spectral analysis of peripheral blood lymphocytes in patients with ovarian cancer (OC) during antitumor treatment.
Methodology Peripheral blood lymphocytes of 20 patients with T3NxMo OC were studied. Patients received endolymphatic polychemotherapy (thiotepa 200 mg, metatrexate 100 mg, 5-fluorouracil 200 mg) twice with a 1-week interval. Lymphocytes isolated during the treatment were stained by the Karnaukhov-Rigler method and studied using a microspectrofluorimeter. Fluorescent spectral analysis was performed.
Results The spectra of OC patients before treatment had only one emission band at 530 nm, with low α parameter values (0.098–0.1 SU). After the beginning of the treatment, a second band appeared on the spectrum at 640 nm, however the band at 530 nm prevailed. During the antitumor treatment and achievement of clinical effect, the intensity of the band at 530 nm significantly decreased on the spectrum in favor of a wavelength of 640 nm. After the end of antitumor therapy, both peaks of approximately the same intensity were present on the spectrum. With the normalization of patients‘ state, α parameter values increased from 0.44 SU to 2.12 SU (p≤0.05). The values were low (0.22–0.048SU) when the state of patients worsened, without clinical effect.
Conclusion The shape of the luminescence spectrum and values of the α parameter vary during the treatment and depend on its results. Luminescent characteristics of peripheral blood lymphocytes allow monitoring the treatment effectiveness and can help to objectively assess the condition of ovarian cancer patients.
Disclosure Nothing to disclose.
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