Introduction/Background Homologous recombination deficiency (HRD) is frequent in non-endometrioid endometrial cancer (EC), justifying clinical trials testing PARP-inhibitors. To facilitate this development a pragmatic test to asses HR competency is required. Accumulation of RAD51 protein at sites of DNA double strand breaks, has been put forward as a reliable functional test for HR competency. Here we aimed to test the performance of this pragmatic assay on formalin-fixed paraffin-embedded (FFPE) tumour tissue.
Methodology We used a previously described, prospective collected series of 21 EC that underwent primary resection with known HR status based on genomic analyses (next-generation sequencing for HR-core genes and array comparative genomic hybridization/SNP array) as our training cohort. To assure the presence of endogenous DNA damage, a gamma-H2AX staining was performed. Co-immunofluorescence (IF) was performed for both Geminin (marker staining cells in G2/S phase) and RAD51. RAD51 score was calculated as the fraction of Geminin positive cells that showed RAD51 foci (≥ 2) in the nucleus. Final RAD51 scores were correlated to known HR-status.
Results All 21 EC showed positivity for gamma-H2AX staining assuring presence of endogenous DNA damage in routine diagnostic FFPE tumour tissue. Among all cases, the RAD51scores varied form 0–72%. The range of RAD51 scores in the five HRD EC was 0–4%, whereas the 16 HRP EC tested showed RAD51 scores ranging from 16–72% (P<0.001).
Conclusion This RAD51 assay can successfully be performed on diagnostic FFPE material and reliably determined HR competency in 100% of our EC cases using cut-off RAD51 score of 10%. We will present the performance of this cut-off on our test-set of HR-annotated EC. This RAD51 assay is a promising cheap, pragmatic assay that can be used in the clinic to select patients that may benefit from platinum compounds and PARP-inhibitor therapy.
Disclosure Nothing to disclose.
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